基于分子印迹技术的电化学传感器检测吡虫啉的研究

Detection of Imidacloprid by Electrochemical Sensor Based on Molecular Imprinting Technology

在玻碳电极上采用循环伏安法沉积金电层,以吡虫啉为模板物质、邻苯二胺(o-PD)为活性单体制备聚合膜。利用循环伏安分析法(CV)、差分脉冲法(DPV)深入研究了印迹传感器的特性,优化聚合过程因素,包括缓冲溶液pH、聚合圈数、洗脱时间、孵育时间。在最佳聚合情况下,其电化学传感器的线性区域是1×10^(-4)~1×10^( -12)mol·L^(-1),检测限为1×10^(-12) mol·L^(-1),加标回收率为104.6%~105.7%,RSD为1.52%~1.65%。

The gold layers were deposited on glassy carbon electrode by cyclic voltammetry.The polymerized gold layers were prepared using imidacloprid as template material and o-phenylenediamine(o-PD)as active monomer.Cyclic voltammetry(CV)and differential pulse method(DPV)were used to further study the characteristics of the imprinted sensor,and the polymerization process factors were optimized,including buffer solution pH,polymerization cycles,elution time and incubation time.Under the optimal polymerization condition,the linear region of the electrochemical sensor was 1×10^(-4)~1×10^(-12) mol·L^(-1),the detection limit was 1×10^(-12) mol·L^(-1),the standard recovery was 104.6%~105.7%,RSD was 1.52%~1.65%.