AIH患儿血清miR-148a表达及其与炎症因子的相关性

Serum miR-148a expression in children with autoimmune hepatitis and its correlation with inflammatory factors

目的探讨自身免疫性肝炎(AIH)患儿血清miR-148a表达及其与炎症因子的相关性。方法选取2018年1月—2021年3月南阳市第一人民医院AIH患儿45例(AIH组)和体检健康儿童53名(正常对照组)。检测所有研究对象血清miR-148a、IgG、炎症因子[转化生长因子β(TGF-β)、白细胞介素17(IL-17)、白细胞介素23(IL-23)]、肝功能[天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、γ-谷氨酰基转移酶(GGT)]和自身抗体[抗核抗体(ANA)、抗肝细胞溶质抗原1型(LC-1)抗体、抗平滑肌抗体(ASMA)、抗肝肾微粒体(LKM)抗体]水平。采用Pearson相关分析评估miR-148a水平与各项指标的相关性。采用Logistic回归分析评估发生AIH的危险因素。结果AIH组血清IgG、AST、ALT、GGT、IL-17、IL-23水平和miR-148a相对表达量,以及ANA、抗LC-1抗体、ASMA、抗LKM抗体阳性率均高于正常对照组(P<0.001),TGF-β水平低于正常对照组(P<0.05)。Pearson相关分析结果显示,AIH患儿miR-148a与IL-17、IL-23、IgG均呈正相关(r值分别为0.504、0.533、0.392,P<0.05),与TGF-β呈负相关(r=-0.527,P<0.05)。miR-148a高表达组ANA、抗LC-1抗体、ASMA、抗LKM抗体阳性率均高于低表达组(P<0.05)。多因素Logistic回归分析结果显示,IL-17、IL-23、miR-148a水平升高和TGF-β水平降低均是发生AIH的独立危险因素(P<0.05)。结论AIH患儿血清miR-148a表达与炎症因子和自身抗体密切相关,其可能参与了AIH的发生。

Objective To investigate serum miR-148a expression in children with autoimmune hepatitis(AIH)and its correlation with inflammatory factors.Methods Totally,45 AIH children(AIH group)and 53 healthy subjects(healthy control group)were enrolled from Nanyang First People's Hospital from January 2018 to March 2021.Serum miR-148a,IgG and inflammatory factors[transforming growth factor-beta(TGF-β),interleukin-17(IL-17)and interleukin-23(IL-23)],liver function[aspartate aminotransferase(AST),alanine aminotransferase(ALT)and gamma-glutamyltransferase(GGT)]and autoantibodies[anti-nuclear antibody(ANA),anti-liver cytosol type 1(LC-1)antibody,anti-smooth muscle antibody(ASMA)and anti-liver and kidney microsome(LKM)antibody]were determined.Pearson correlation analysis was used to evaluate the correlation between miR-148a levels and various indicators.Logistic regression analysis was used to evaluate the risk factors of AIH occurrence.Results In AIH group,the levels of serum IgG,AST,ALT,GGT,IL-17 and IL-23,the relative expression of miR-148a and the positive rates of ANA,anti-LC-1 antibody,ASMA and anti-LKM antibody were higher than those in healthy control group(P<0.001).TGF-β was lower than that in healthy control group(P<0.05).Pearson correlation analysis showed that miR-148a was positively correlated with IL-17,IL-23 and IgG(r=0.504,0.533 and 0.392,respectively,P<0.05),and there was a negative correlation with TGF-β(r=-0.527,P<0.05).The positive rates of ANA,anti-LC-1 antibody,ASMA and anti-LKM antibody in miR-148a high expression group were higher than those in miR-148a low expression group(P<0.05).Multivariate Logistic regression analysis showed that the increased levels of IL-17,IL-23 and miR-148a and the decreased level of TGF-βwere independent risk factors for AIH occurrence(P<0.05).Conclusions Serum miR-148a in children with AIH is related to inflammatory factors and autoantibodies,which may be involved in AIH occurrence.