小米多肽酶解工艺及其抗氧化和ACE抑制活性

Enzymatic Hydrolysis Process of Millet Polypeptides and Its Antioxidantand ACE Inhibitory Activities

以小米蛋白为原料,制备具有抗氧化活性和血管紧张素转移酶(ACE)抑制活性的食源性多肽。采用碱性蛋白酶、中性蛋白酶和风味蛋白酶酶解小米蛋白,选取最优酶解工艺。选用碱性蛋白酶酶解4 h,得到的蛋白水解液分别通过3 ku和10 ku的超滤膜,得到分子质量<3,3~10 ku和>10 ku的3种组分,测定其抗氧化活性和ACE抑制活性。结果表明:采用碱性蛋白酶酶解4 h得到的蛋白水解液经超滤后得到分子质量<3 ku的多肽具有最高的抗氧化活性和ACE抑制活性,其DPPH清除能力为38.44%,ABTS自由基清除能力为81.62%,羟基自由基清除能力为68.49%,ACE抑制活性为87.53%。此方法得到的多肽分子质量小,功能氨基酸含量较高,具有良好的抗氧化活性和ACE抑制活性。本研究结果为抗氧化肽和ACE抑制肽的开发提供试验依据,对未来工业化的发展提供理论参考。

Millet protein was used as raw material to prepare food-derived peptides with antioxidant activity and an giotensin transferase(ACE)inhibitory activity.Alkaline protease,neutral protease and flavor protease were used to hydrolyze millet protein.Three components with molecular weight<3,3-10 ku and>10 ku were obtained from the hydrolysate obtained by alkaline protease for 4 h,and their antioxidant and ACE inhibitory activities were determined.The results showed that the polypeptides with molecular weight<3 ku obtained from the hydrolysate obtained by alkaline protease for 4 h after ultrafiltration had the highest antioxidant activity and ACE inhibitory activity,with DPPH scavenging capacity of 38.44%,ABTS free radical scavenging capacity of 81.62%,hydroxyl radical scavenging capacity of 68.49%.The inhibitory activity of ACE was 87.53%.The polypeptides obtained by this method have low molecular weight,high content of functional amino acids,and have good antioxidant and ACE inhibitory activities,which provide a basic for the development of antioxidant peptides and ACE inhibitory peptides,and provide a theoretical basis for the future industrial development.