灰树花水上清液中不同极性提取物的功能成分及其抗氧化活性

Functional Components and Antioxidant Activities of Different Polarities Extracts from the Supernatant of Grifola frondosa

分别采用石油醚、乙酸乙酯、正丁醇、水不同极性溶剂提取灰树花水提上清液,比较不同极性提取物的主要功能成分及利用1,1-二苯基-2-三硝基苯肼(DPPH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS+)、羟基(OH)自由基3种方法比较抗氧化活性。结果表明,正丁醇提取物的多酚和蛋白质含量最高,分别为0.36%和38.75%;石油醚和乙酸乙酯提取物中黄酮含量最高,均为0.05%;石油醚提取物的三萜、甾醇含量最高,分别为6.32%和15.88%;水提取物中多糖含量最高,为30.11%。正丁醇提取物具有最强的抗氧化活性,多酚是主要起抗氧化活性的成分。通过高效液相色谱质谱联用法(LC-MS)鉴定了13个多酚及其衍生物。本研究为灰树花功能性食品或保健品的开发提供了理论依据。

In the study,petroleum ether,ethyl acetate,n-butanol and water with different polar solvents were used to the water-extracted supernatant of Grifola frondosa.The main functional components of different polar extracts were compared,and the antioxidant activities were compared by three methods:1,1-Diphenyl-2-trinitrophenylhydrazine(DPPH),2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diammonium salt(ABTS+),hydroxyl(OH)free radical.The results showed that the polyphenol and protein contents of the n-butanol extract were the highest at 0.36%and 38.75%,respectively.The highest flavonoid content in petroleum ether and ethyl acetate extracts was 0.05%.The contents of triterpenoids and sterols in petroleum ether extract were 6.32%and 15.88%,respectively.The highest polysaccharide content in water extract was 30.11%.N-butanol extract showed the strongest antioxidant activity,and polyphenols may be the main component of antioxidant activity.Thirteen polyphenols and their derivatives were identified by LC-MS.This provides a theoretical basis for the development of Grifola frondosa functional food or health products.