豌豆蚜几丁质脱乙酰酶(ApCDAs)的鉴定及表达特性

Identification and Expression of Aphid Chitin Deacetylases(ApCDAs)in Acyrthosiphon pisum

通过BLAST比对和软件分析,共筛选出4个豌豆蚜(Acyrthosiphon pisum)几丁质脱乙酰基因( ApCDA1、 ApCDA2、 ApCDA3和 ApCDA5),并分析了其生物学信息;通过实时荧光定量PCR技术,发现4个ApCDAs在各发育阶段均有表达,其中, ApCDA1基因在1龄、2龄若虫和成虫期表达量较高;ApCDA2基因在4龄若虫的表达量高于其他龄期;ApCDA3基因在各发育阶段后期的表达量显著高于前期和中期,2龄若虫后期表达量最高;ApCDA5基因在3龄若虫的表达量偏高,2龄后期的表达量最高;此外, ApCDA1和 ApCDA3在3龄若虫和成虫中肠、胚胎和表皮内的表达量均显著高于 ApCDA2和 ApCDA5。结果表明,ApCDAs基因对豌豆蚜的生长发育和蜕皮具有重要作用。

Through BLAST and software analysis,we identified four chitin deacetylation genes(ApC-DA1,ApCDA2,ApCDA3 and ApCDA5)in Acyrthosiphon pisum,and analyzed their biological char-acteristics.Real-time PCR indicated that all four ApCDAs were expressed at developmental stages.ApCDA1 exhibited higher expression in first instar nymphs,second instar nymphs and adults,while ApCDA2 displayed higher expression in the fourth instar nymphs compared to other instars.The ex-pression of ApCDA3 was significantly higher at the late stage of development than at the early and middle stages,with peak expression occurring at the late stage of the second instar nymphs.Further-more,the expressions of ApCDA1 and ApCDA3 were significantly higher than those of ApCDA2 and ApCDA5 in the midgut,embryo and epidermis of third instar nymphs and adults.In summary,ApC-DAs play a key role in the growth and development of pea aphid.