肾动脉缺血再灌注诱导急性肾损伤模型的建立和评价

Establishment and Evaluation of Renal Artery Ischemia-reperfusion-induced Acute Kidney Injury Model

目的:观察缺血再灌注诱导急性肾损伤大鼠模型的特点。方法:采用双侧肾动脉夹闭的方法建立急性肾损伤大鼠模型,采用全自动生化分析、Elisa检测、HE染色等方法,检测血肌酐、尿素、胱抑素C及肾脏病理损伤情况。结果:缺血再灌注处理可诱导SD大鼠的肾脏指数升高,血清肌酐、尿素、胱抑素C的水平显著升高,肾脏损伤评分明显升高,且肾脏病理主要表现为肾小管损伤。结论:双侧肾动脉夹闭的方法,可引起大鼠肾功能急性损伤,病理损伤主要表现为急性肾小管的损伤,可成功建立IR-AKI大鼠模型,且模型较稳定,AKI特征明显。

Objective:To observe the model characteristics of ischemia-reperfusion-induced acute kidney injury in rats.Methods:We established a rat model of acute kidney injury by bilateral renal artery clipping,and used the methods of biochemical analysis,Elisa detection and HE staining to detect the levels of serum creatinine,urea,cystatin C and the pathological damage of the kidneys.Results:Ischemia-reperfusion treatment can significantly increase the renal index,the levels of serum creatinine,urea,cystatin C and renal injury score in SD rats.And the renal pathology is mainly manifested as renal tubular damage.Conclusion:The method of bilateral renal artery clipping can cause acute decline of renal function in rats,and the pathological damage is mainly manifested as acute renal tubular damage.Through this method,the IR-AKI rat model can be successfully established,and the model is relatively stable and has obvious characteristics of AKI.