桥本甲状腺炎患者血清miR-326、miR-155与甲状腺相关抗体和Th17/Treg细胞因子失衡的相关性分析

Correlation Analysis of Serum miR-326 and miR-155 with Thyroid-associated Antibodies and Th17/Treg Cytokine Imbalance in Patients with Hashimoto Thyroiditis

目的:探讨桥本甲状腺炎(HT)患者血清微小核糖核酸(miR)-326、miR-155与甲状腺相关抗体和辅助性T细胞17(Th17)/调节性T细胞(Treg)细胞因子失衡的相关性。方法:选择2021年1月至2022年10月空军军医大学唐都医院收治的HT患者82例作为研究组,同期接受体检的健康人80例作为对照组,比较两组血清miR-326、miR-155、甲状腺功能参数[促甲状腺激素(TSH)、游离三碘甲状原氨酸(FT_(3))、游离甲状腺素(FT_(4))]、甲状腺相关抗体[甲状腺过氧化物酶抗体(TPOAb)、甲状腺球蛋白抗体(TGAb)]、Th17、Treg水平及其细胞因子水平,并分析HT患者血清miR-326、miR-155与甲状腺相关抗体和Th17/Treg细胞因子失衡的相关性。结果:研究组TSH、TPOAb、TGAb显著高于对照组,FT_(3)、FT_(4)显著低于对照组(P<0.05)。研究组血清miR-326、miR-155表达水平显著高于对照组(P<0.05)。研究组Th17、Th17/Treg、白细胞介素-17(IL-17)、γ干扰素(IFN-γ)显著高于对照组,Treg、白细胞介素-10(IL-10)水平显著低于对照组(P<0.05)。Pearson相关分析显示,HT患者血清miR-326、miR-155分别与TSH、TPOAb、TGAb、Th17、Th17/Treg、IL-17、IFN-γ呈正相关,与FT_(3)、FT_(4)、Treg、IL-10呈负相关(P<0.05)。结论:HT患者血清miR-326、miR-155水平异常升高,其水平与TPOAb、TGAb自身抗体及Th17/Treg失衡相关,血清miR-326、miR-155可能通过影响Th17/Treg免疫平衡,促进HT的发生和发展。

Objective:To investigate the correlation between serum micro ribonucleic acid(miR)-326 and miR-155 with thyroid-associated antibodies and T helper cell 17(Th17)/regulatory T cell(Treg)cytokine imbalance in patients with hashimoto thyroiditis(HT).Method:82 HT patients who were admitted to Tangdu Hospital of Air Force Military Medical University from January 2021 to October 2022 were selected as study group,and 80 healthy individuals who underwent physical examinations as control group during the same period,the levels of serum miR-326,miR-155,thyroid function parameters[thyroid stimulating hormone(TSH),free triiodothyronine(FT_(3)),free thyroxine(FT_(4))],thyroid-associated antibodies[thyroid peroxidase antibody(TPOAb),thyroglobulin antibody(TGAb)],Th17,Treg and their cytokine levels were compared between two group,the correlation between serum miR-326,miR-155 and thyroid-associated antibodies and Th17/Treg cytokine imbalance in HT patients were analyzed.Results:TSH,TPOAb and TGAb in study group were significantly higher than those in control group,FT_(3) and FT_(4) were significantly lower than those in control group(P<0.05).The expression levels of serum miR-326 and miR-155 in study group were significantly higher than those in control group(P<0.05).Th17,Th17/Treg,interleukin-17(IL-17)and interferon-γ(IFN-γ)in study group were significantly higher than those in control group,the levels of Treg and interleukin-10(IL-10)were significantly lower than those in control group(P<0.05).Pearson correlation analysis showed that,the serum miR-326 and miR-155 in HT patients were positively correlated with TSH,TPOAb,TGAb,Th17,Th17/Treg,IL-17 and IFN-γrespectively,and were negatively correlated with FT_(3),FT_(4),Treg and IL-10(P<0.05).Conclusion:The levels of serum miR-326 and miR-155 in HT patients are abnormally increase,which level is relate to TPOAb,TGAb autoantibodies and Th17/Treg imbalance,serum miR-326 and miR-155 may affect Th17/Treg immune balance,and promote the occurrence and development of HT.