实验教学用植物活体荧光影像系统的研制

Research and Development of Living Plants in-vivo Fluorescence Imaging System for Experimental Teaching

通过植物培养及拍摄系统的开发,研制低成本的植物活体荧光影像系统。该系统采用双层箱体结构设计,分为恒温光照培养系统和荧光记录系统两部分,可以为植物生长发育提供所需的生命支撑功能,还能实时获取植物生长发育过程中的关键图像,包括监控转基因植物发育过程中荧光信号的时空模式。利用该系统成功地对EGFP转基因烟草和拟南芥进行培养和实时观察。低廉的成本极大地扩展了该系统在实验教学中的运用,也为生命活动的观察和检测研究奠定基础。

Through the development of plant culture and imaging system,a low-cost plant living fluorescence imaging system was developed.The system adopts a double-layer box structure design,which is divided into two parts:the constant culture system and the fluorescence recording system.It can provide the necessary life support function for plant growth,and can also obtain the key images in the process of plant growth in real time,including the time-space mode of monitoring the fluorescence signal in the development of the transgenic plant.The EGFP transgenic tobacco and Arabidopsis were successfully cultured and observed in real time using this system.The low cost greatly expanded the application of the system in experimental teaching,and also laid a foundation for the further study of the observation and detection of follow-up life activities.