不同地区桃树根际土壤微生物群落结构及多样性分析

Microbial Community Structure and Diversity of Peach Rhizosphere Soil in Different Regions

植物根际土壤微生物群落能够加速根际营养元素的循环,调节土壤肥力。分析桃树根际土壤微生态结构,可以为桃树种植土壤改良提供依据。利用高通量测序技术对临沂市李官、汤头、沂水3个地区桃园根际土壤微生物多样性和群落组成进行分析。经筛选和去除嵌合体后,得到了425267条优质序列,这些序列的长度主要集中在400~450 bp。在3个地区的样品中共鉴定出4175个操作性分类单元(OTU)。物种分类结果显示,细菌包括25个门、54个纲、145个目、272个科、497个属和577个物种。在临沂不同地区的桃树根际土壤样品中,细菌群落组成和结构存在一定差异,细菌丰富度和多样性的顺序为汤头>沂水>李官。细菌群落中,线菌门和变形菌门的丰度之和达到50.37%~66.07%,属于绝对优势菌门。根据COG功能分类统计,土壤细菌的功能基因主要集中在新陈代谢、遗传信息和细胞信号传导等方面。总体而言,桃根际土壤的细菌多样性和群落组成在不同地区之间存在差异。

Plant rhizosphere soil microbial community can accelerate the circulation of rhizosphere nutrients and regulate soil fertility.The analysis of rhizosphere soil microecological structure of peach trees can effectively improve the planting soil of peach trees.The rhizosphere soil microbial diversity and community composition of peach orchard in Liguan,Tangtou and Yishui of Linyi city were analyzed by high-throughput sequencing technology.After screening and removal of chimeras,425267 high-quality sequences were obtained,and the length of these sequences was mainly concentrated in 400-450 bp.A total of 4175 operational taxa(OTU)were identified in samples from the three regions.The species classification showed that bacteria included 25 phyla,54 classes,145 orders,272 families,497 genera and 577 species.In the rhizosphere soil samples of peach trees in different areas of Linyi,there were some differences in the composition and structure of bacterial communities,and the order of bacterial richness and diversity was Tangtou>Yishui>Liguan.In the bacterial community,the sum of abundance of Actinobacteria and Proteobacteria reached 50.37%-66.07%,belonging to the absolute dominant bacteria.According to COG functional classification statistics,functional genes of soil bacteria mainly focus on metabolism,genetic information and cell signal transduction.In general,the bacterial diversity and community composition of peach rhizosphere soil were different in different regions.