苍附导痰丸治疗痰湿型多囊卵巢综合征的疗效及对外周血PI3K/AKT信号通路的影响

Efficacy of Cangfu Daotan Pill on Phlegm Dampness Type Polycystic Ovary Syndrome and Its Influence on the PI3K/AKT Signaling Pathway in Peripheral Blood

目的:探讨苍附导痰丸治疗痰湿型多囊卵巢综合征(PCOS)的疗效及对外周血磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(AKT)信号通路的影响。方法:纳入河北省沧州中西医结合医院2022年1月~2022年10月收治的86例痰湿型PCOS患者。根据随机数字表法,分为对照组(n=43,达英-35治疗)和实验组(n=43,达英-35联合苍附导痰丸治疗)。观察两组中医证候积分、性激素指标、糖脂代谢指标、PI3K/AKT信号通路相关指标变化情况和不良反应发生率。结果:与对照组治疗后相比,实验组中医证候积分、甘油三酯(TG)、促卵泡生成素(FSH)、低密度脂蛋白胆固醇(LDL-C)、睾酮(T)、促黄体生成素(LH)、空腹血糖(FBG)、总胆固醇(TC)、空腹胰岛素(FINS)水平和PI3K信使核糖核酸(mRNA)、AKT mRNA更低,高密度脂蛋白胆固醇(HDL-C)更高(P<0.05)。两组不良反应发生率对比未见差异(P>0.05)。结论:苍附导痰丸治疗痰湿型PCOS患者,可有效调节性激素、糖脂代谢指标,改善临床症状,可能与调节外周血PI3K/AKT信号通路有关。

Objective:To explore the efficacy of Cangfu Daotan Pill on phlegm dampness type polycystic ovary syndrome(PCOS)and its influence on the phosphatidylinositol-3-kinase(PI3K)/protein kinase B(AKT)signaling pathway in peripheral blood.Methods:86 patients with phlegm dampness type PCOS who were admitted to Cangzhou Integrated Traditional Chinese and Western Medicine Hospital of Hebei Province from January 2022 to October 2022 were included.According to the random number table method,they were divided into control group(n=43,treated with Diane-35)and experimental group(n=43,treated with Diane-35 combined with Cangfu Daotan Pill).The traditional Chinese medicine syndrome scores,sex hormone indicators,glucose and lipid metabolism indicators,PI3K/AKT signaling pathway related indicators changes and the incidence of adverse reactions were observed in two groups.Results:Compared with control group after treatment,experimental group had lower traditional Chinese medicine syndrome scores,triglyceride(TG),follicle stimulating hormone(FSH),low-density lipoprotein cholesterol(LDL-C),testosterone(T),luteinizing hormone(LH),fasting blood glucose(FBG),total cholesterol(TC),fasting insulin(FINS)levels and lower PI3K messenger ribonucleic acid(mRNA),AKT mRNA,and higher high-density lipoprotein cholesterol(HDL-C)(P<0.05).There was no difference in the incidence of adverse reactions between two groups(P>0.05).Conclusion:Cangfu Daotan Pill is used to treat patients with phlegm dampness type PCOS,which can effectively regulate sex hormones and glucose and lipid metabolism indicators,improve clinical symptoms,may be related to regulating the PI3K/AKT signaling pathway in peripheral blood.