粗毛纤孔菌不同提取物对免疫抑制小鼠免疫功能的影响

Effects of Different Extracts from Inonotus hispidus on Immune Function in Immunosuppressed Mice

采用粗毛纤孔菌(Inonotus hispidus)石油醚提取物(高、低剂量分别为12、6 mg·kg^(-1))、乙酸乙酯提取物(高、低剂量组分别为17、8.5 mg·kg^(-1))、正丁醇提取物(高、低剂量分别为45、22.5 mg·kg^(-1))、水提取物(高、低剂量分别为600、300 mg·kg^(-1))、麦角甾醇(高、低剂量分别为10、5 mg·kg^(-1))灌胃环磷酰胺所致免疫抑制小鼠,测定免疫器官指数、吞噬指数、吞噬系数、耳肿胀度、耳肿胀率和血清细胞因子、免疫球蛋白含量,采用苏木素-伊红染色小鼠脾脏、胸腺、胰腺、回肠后观察组织形态,研究粗毛纤孔菌不同提取物对免疫抑制小鼠免疫功能的影响。结果表明:与模型组相比,石油醚、水提取物高剂量组和麦角甾醇高、低剂量组的胸腺指数显著升高;麦角甾醇高、低剂量组的吞噬指数和吞噬系数均显著升高,水提取物高、低剂量组的吞噬系数显著升高;水提取物和麦角甾醇高、低剂量组的耳肿胀度和耳肿胀率均极显著升高;石油醚、水提取物和麦角甾醇高、低剂量组,乙酸乙酯提取物高剂量组的IL-1β含量显著增加;石油醚、乙酸乙酯、水提取物高、低剂量组和麦角甾醇高、低剂量组的IL-2含量极显著增加;除正丁醇提取物低剂量组外,其余提取物组和麦角甾醇高、低剂量组的IFN-γ和IL-10含量均极显著增加;石油醚、水提取物和麦角甾醇高、低剂量组,乙酸乙酯提取物高剂量组的IL-4含量极显著增加;石油醚、水提取物和麦角甾醇高、低剂量组的IgG含量显著增加。研究结果表明,粗毛纤孔菌提取物对环磷酰胺所致免疫抑制小鼠具有良好的免疫保护作用,可为粗毛纤孔菌进一步开发提供参考。

Different solvents were used to extract Inonotus hispidus and then the resultant extracts were given to cyclophosphamide induced immunosuppressed mice by gavage respectively.The extracts included petroleum ether extract at high(12 mg·kg^(-1))and low(6 mg·kg^(-1))doses,ethyl acetate extract at high(17 mg·kg^(-1))and low(8.5 mg·kg^(-1))doses,n-butanol extract at high(45 mg·kg^(-1))and low(22.5 mg·kg^(-1))doses,water extract at high(600 mg·kg^(-1))and low(300 mg·kg^(-1))doses,and purified ergosterol at high(10 mg·kg^(-1))and low(5 mg·kg^(-1))doses.Mice in different treatment groups were measured for immune organ indices,phagocytic index,phagocytic coefficient,ear swelling degree,ear swelling rate,and contents of cytokines and immunoglobulins.Hematoxylineosin staining was used to observe the morphology of spleen,thymus,pancreas and ileum tissue of mice under different treatments.The results showed that thymus index was significantly increased in the high dose petroleum ether extract group,the high dose water extract group and the purified ergosterol groups as compared with the model group.Both phagocytic index and phagocytic coefficient were significantly increased in the purified ergosterol groups,and the water extract increased phagocytic coefficient.The purified ergosterol and water extract also extremely significantly increased ear swelling degree and ear swelling rate.The content of IL-1βwas significantly increased in the petroleum ether extract,water extract,ergosterol,and the high dose ethyl acetate extract groups.Similarly,the content of IL-2 was significantly increased in mice treated with the petroleum ether extract,ethyl acetate extract,water extract,and ergosterol.Except the low-dose n-butanol extract group,all the other extract groups and the purified ergosterol groups showed a significant increase in IFN-γand IL-10 contents.The content of IL-4 was extremely significantly increased in mice treated with the petroleum ether extract,water extract,ergosterol and high dose ethyl acetate extract.The petroleum ether extract,water extract and ergosterol also significantly increased the content of IgG.The extracts of I.hispidus showed a good immune protection effect on cyclophosphamide induced immunosuppressed mice,and thus provided a reference for the further development of I.hispidus.