摘要
为评价茯苓(Poria cocos)多糖对非小细胞肺癌NCI-H460细胞的抑制作用,用CCK-8法测定茯苓多糖对细胞增殖的抑制率,用DAPI染色法、AOEB双荧光染色法分析茯苓多糖对细胞凋亡的影响,用细胞划伤愈合实验测定茯苓多糖对细胞迁移能力的影响,用蛋白免疫印迹实验检测茯苓多糖对细胞凋亡、迁移相关蛋白表达的影响。结果表明:茯苓多糖具有抑制NCI-H460细胞增殖、集落形成和迁移的能力,可诱导NCI-H460细胞凋亡;500、1000μg·mL^(-1)的茯苓多糖可使Bax和P53蛋白表达量升高,Bcl-2和MMP-2蛋白表达量降低。
The inhibitory effect of Poria cocos polysaccharide(PCP)polysaccharide on non-small-cell lung cancer NCI-H460 cells was studied.CCK-8 kit was used to determine the inhibition rate of PCP on NCI-H460 cell proliferation.DAPI staining and AOEB double fluorescence staining were used to analyze the effect of PCP on apoptosis in NCI-H460 cells.The scratch assay was used to evaluate the effect of PCP on cell migration.Western blotting was used to detect the effect of PCP on protein expression pertaining to apoptosis and migration.The results showed that PCP effectively inhibited the proliferation,colony formation and migration of NCI-H460 cells,and induced cell apoptosis.At 500 and 1000μg·mL^(-1),PCP significantly increased the expression of Bax and P53,but inhibited the expression of Bcl-2 and MMP-2 in NCI-H460 cells.
作者
孙恺婧
刘馨泽
孙莹
金鑫
李琼
陈长宝
万茜淋
SUN Kaijing;LIU Xinze;SUN Ying;JIN Xin;LI Qiong;CHEN Changbao;WAN Xilin(Jilin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,Jilin,China;Clinical Laboratory,the Second Hospital of Dalian Medical University,Dalian 116027,Liaoning,China;Key Laboratory of Molecular Epigenetics,Northeast Normal University,Ministry of Education,Changchun 130021,Jilin,China)
出处
《食用菌学报》
CSCD
北大核心
2024年第1期76-83,共8页
Acta Edulis Fungi
基金
吉林省科学技术厅吉林省科技发展计划项目(20230508166RC)
吉林省教育厅科学技术研究项目(JJKH20231312KJ)。
关键词
茯苓多糖
非小细胞肺癌
NCI-H460细胞
细胞增殖
细胞迁移
Poria cocos polysaccharide(PCP)
non-small-cell lung cancer
NCI-H460 cell
cell proliferation
cell migration
