胰激肽原酶对背部皮瓣损伤大鼠皮瓣存活及炎症因子与氧化应激的影响

Effects of Pancreatic Kallikrein on Flap Survival,Inflammatory Factors and Oxidative Stress in Rats with Dorsal Flap Injury

目的:探讨胰激肽原酶对大鼠背部超长随意皮瓣存活、炎症因子和氧化应激的影响。方法:将SPF级SD大鼠分为假手术组、生理盐水组、胰激肽原酶低剂量组和胰激肽原酶高剂量组。假手术组仅切开皮瓣,其余组做皮瓣损伤模型,生理盐水组不给予药物处理,胰激肽原酶低剂量组给予7.2 U/kg胰激肽原酶,胰激肽原酶高剂量组给予14.4 U/kg胰激肽原酶,治疗7 d。实验结束后,观察大鼠皮瓣成活率,HE染色观察大鼠皮瓣病理变化,使用原位末端转移酶标记技术(Terminaldeoxynucleoitidyl transferase mediated nick end labeling,TUNEL)染色观察皮瓣细胞凋亡情况,CD31染色观察血管形成情况,ELISA检测大鼠血清中TNF-α和IL-6含量,皮瓣中Nrf2/HO-1水平。结果:与生理盐水组相比胰激肽原酶组提高了大鼠皮瓣存活率,减轻了皮瓣组织损伤,降低了血清TNF-α和IL-6含量,增高了Nrf2/HO-1水平。结论:胰激肽原酶能够提升皮瓣存活率,减轻大鼠炎症反应和氧化应激反应。

Objective To investigate the effects of pancreatic kininogenase on the survival,inflammatory factors and oxidative stress of super-long random skin flap in rats.Methods SPF SD rats were divided into sham operation group,control group,low dose pancreatic kininogenase group and high dose pancreatic kininogenase group.The sham operation group only cut open the skin flap,the other groups made the skin flap injury model,the control group was not given drug treatment,the pancreatic kininogenase low dose group was given 7.2 U/kg pancreatic kininogenase,and the pancreatic kininogenase high dose group was given 14.4 U/kg pancreatic kininogenase for 7 days.At the end of the experiment,the survival rate of rat skin flap was observed,the pathological changes of rat skin flap were observed by HE staining,the apoptosis of skin flap was observed by TUNEL staining,the angiogenesis was observed by CD31 staining,the contents of TNF-αand IL-6 in serum and the level of Nrf2/HO-1 in skin flap were detected by ELISA.Results compared with the control group,pancreatic kininogenase increased the survival rate of skin flap,reduced the tissue injury of skin flap,decreased the content of serum TNF-αand IL-6,and increased the level of Nrf2/HO-1.Conclusion pancreatic kininogenase can improve the survival rate of skin flap and reduce inflammatory reaction and oxidative stress in rats.