灵菊七蛋白提取及其降糖活性研究

Study on extraction and hypoglycemic activity of protein from Gynura medica

为了开发降糖药用植物灵菊七,从提取溶剂、加热方式、提取时间、提取温度、料液比5个方面对灵菊七活性蛋白提取工艺进行了研究,并检测了不同工艺获得的提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用,通过响应面法优化了提取条件.结果表明,蛋白提取率最高的提取条件为:提取温度62℃、提取时间3 h、料液比1∶30、提取溶剂PBS缓冲液,蛋白最大提取率可达10.8%;提取液对α-淀粉酶具有最高抑制率的提取条件为:提取温度59℃、提取时间3 h、料液比1∶30、提取溶剂PBS缓冲液,最大抑制率为34.49%;浸提液对α-葡萄糖苷酶具有最高抑制率的提取条件为:提取温度52℃、提取时间5 h、料液比1∶30、提取溶剂PBS缓冲液,灵菊七浸提液对α-葡萄糖苷酶的最大抑制率达到16.04%.浸提液对α-淀粉酶抑制率随着蛋白含量的增加而升高,结合提取液蛋白含量和纯度研究,可以推测灵菊七浸提液含有对α-淀粉酶具有较好抑制作用的活性蛋白.

In order to develop the hypoglycemic medicinal plant named Gynura medica,the extraction process of the active protein of Gynura medica was studied in terms of extraction solvent,heating method,extraction time,extraction temperature and material liquid ratio,and the effects of the extracts obtained by different processes onα-Amylase andα-glucosidase was detected.The extraction conditions were optimized by response surface methodology.The results showed that the maximum extraction rate of protein was 10.8%under the following conditions:extraction temperature 62℃,extraction time 3 h,material liquid ratio 1∶30,extraction solvent PBS.The extraction conditions of the extractor with the highest inhibition rate ofα-amylase were as follows:extraction temperature 59℃,extraction time 3h,material liquid ratio 1∶30,extraction solvent PBS,the maximum inhibition rate was 31%;The extraction conditions of the extractor with the highest inhibition rate ofα-glucosidase were as follows:extraction temperature 52℃,extraction time 5 h,material liquid ratio 1∶30,extraction solvent PBS.The maximum inhibition rate ofα-glucosidase was 13.60%.The inhibition rate ofα-amylase increased with the increase of protein content.Considering the protein content and purity of the extract,it can be inferred that the extractor of Gynura medica contained a highα-amylase inhibition activity protein.