不同培养时间金针菇栽培基质的饲用营养价值评定

Evaluation on nutritional value of Flammulina filiformis cultivation substrate with different cultivation time

为评定培养时间对金针菇(Flammulina filiformis)栽培基质饲用营养价值的影响,在金针菇栽培的第0、9、14、18、22、29、36、51天收集金针菇栽培基质,测定基质中的营养成分含量与氨基酸组成,并采用尼龙袋法测定在瘤胃中进行体内消化0、2、4、8、12、24、48、72 h后样品的干物质消失率,及24 h和48 h的营养物质降解率。结果表明,与处理前相比,培养29 d的基质中,粗蛋白含量显著(P<0.05)提升至8.96%,必需氨基酸和总氨基酸含量分别显著提升至0.47%和1.01%,酸性洗涤木质素含量显著降至试验条件下的最低水平(7.72%)。培养51 d的基质中,半纤维素含量达31.37%,是各处理中最高的,且有较高的干物质消失率。综上,金针菇栽培基质可作为反刍动物的饲料资源,最适利用时间为培养29 d。

In order to evaluate the effect of different cultivation time on the nutritional value of Flammulina filiformis cultivation substrate,the samples of the cultivation substrate of F.filiformis were collected on the 0th,9th,14th,18th,22nd,29th,36th,and 51st days of cultivation.The contents of nutritional ingredients and amino acid(AA) composition of these samples were determined.Besides,the nylon bag technique was adopted to determine the dry matter degradability after in vivo digestion in the rumen for 0,2,4,8,12,24,48,72 h,respectively,as well as the degradation rate of nutritional ingredients after in vivo digestion for 24,48 h,respecitvely.It was shown that,compared with the substrate before cultivation,the contents of crude protein,essential amino acids and total amino acids in the substrate cultivated for 29 d were significantly(P<0.05) increased to 8.96%,0.47%,1.01%,respectively,yet the aicd detergent lignin content was significantly decreased to the lowest level(7.72%) under the experiement conditions.For the substrate cultivated for 51 d,the hemicellulose content was the highest(31.37%),and the dry matter degradability was relatively high.In general,F.filiformis cultivation substrate could be used as the feed resource for ruminants,and the optimized cultivation time was 29 d.