7个匍匐翦股颖抗褐斑病的初步比较及生理响应

Preliminary comparison and physiological response of 7 varieties of creeping bentgrass against brown spot disease

为筛选抗病匍匐翦股颖材料,本实验对立枯丝核菌接菌侵染后的7个匍匐翦股颖材料的生理指标进行测定,通过主成分分析对其抗病性进行综合比较。结果表明,受到病原菌入侵后,植物叶片的叶绿素、可溶性蛋白(SP)、过氧化氢(H_(2)O_(2))含量下降;丙二醛(MDA)、可溶性糖(SS)、游离脯氨酸(Pro)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽还原酶(APX)含量上升。将这10个生理指标转化为4个主成分因子,计算出得分D值,评分最高的为‘光芒’。通过主成分分析法综合各项生理指标表明:7个品种匍匐翦股颖的抗病性排序为光芒>A-1>007>A4>朝阳>宣言>佳美,为后续匍匐翦股颖抗病机制的研究以及抗病新品种的培育奠定基础。

In order to screen the resistant varieties of creeping bentgrass,the physiological indexes of 7 varieties of creeping bentgrass infected by Rhizoctonia solani were determined and their disease resistance was compared by principal component analysis.The results showed that after being invaded by pathogens,the content of chlorophyll,soluble protein(SP),and hydrogen peroxide(H_(2)O_(2))in plant leaves decreased;the content of malondialdehyde(MDA),soluble sugar(SS),free proline(Pro),superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),and glutathione reductase(APX)increased.the score D value was calculated following conversion of these 10 physiological indicators into 4 principal component factors,The highest score D went to factor was‘Guangmang’.The disease resistance level of 7 varieties of creeping bentgrass was ranked as Guangmang>A-1>007>A4>Sun Rise>Xuanyan>Jiamei by principal component analysis.The results laid the foundation for the subsequent research on the resistance mechanism of creeping bentgrass and the breeding of new resistant varieties.