桂枝加龙骨牡蛎汤对魄不安于肺不寐大鼠脑组织Caspase-3、Bcl-2、Bax水平的影响

Effects of Cinnamon Twig Decoction Plus Eragon Bone and Oyster Shell on the Levels ofCaspase-3, Bcl-2 and Bax in Brain Tissue of Rats with Insomnia Due toCorporeal Soul Restless in the Lung

目的:探讨桂枝加龙骨牡蛎汤对魄不安于肺不寐大鼠脑组织Caspase-3、Bcl-2、Bax表达的影响及其治疗魄不安于肺不寐可能的作用机制。方法:32只雄性SD大鼠随机分为对照组、模型组、中药组和西药组,每组8只。采用水环境小平台法干预9 d建立魄不安于肺不寐大鼠模型,造模成功后,中药组予以桂枝加龙骨牡蛎汤7.6 g·kg-1·d-1灌胃,西药组予以右佐匹克隆片0.1 mg·kg-1·d-1灌胃,对照组和模型组给予等体积生理盐水灌胃,连续14 d,测量体质量。采用免疫组织化学法及蛋白质免疫印迹法检测脑组织中Caspase-3、Bcl-2、Bax表达水平。结果:与对照组比较,模型组大鼠体质量减轻(P<0.01),免疫组化检测结果显示,大鼠脑组织Bcl-2的表达显著减少(P<0.01),Caspase-3、Bax表达显著增加(P<0.01),Bcl-2/Bax比率显著减少(P<0.01);Western blot结果显示大鼠脑组织Bcl-2相对表达量显著减少(P<0.01),Bax、Caspase-3相对表达量显著增加(P<0.01)。与模型组比较,中药组及西药组大鼠体质量显著增加(P<0.01),免疫组化检测结果显示,大鼠脑组织Bcl-2表达增加(P<0.05),Caspase-3、Bax表达减少(P<0.05),Bcl-2/Bax比率显著升高(P<0.01);Western blot结果显示大鼠脑组织中Bcl-2相对表达量增加(P<0.05),Bax、Caspase-3相对表达量显著减少(P<0.01)。结论:桂枝加龙骨牡蛎汤改善魄不安于肺不寐大鼠的睡眠可能与增加其脑组织Bcl-2、降低Caspase-3、Bax表达水平有关。

Objective:To investigate the effects of cinnamon twig decoction plus eragon bone and oyster shell(CDEO)on the levels of Caspase-3,Bcl-2 and Bax in brain tissue of rats with insomnia due to corporeal soul restless in the lung and its possible mechanism in the treatment of the disease.Methods:Thirty-two male SD rats were randomly divided into control group,model group,traditional Chinese medicine group and Western medicine group,with 8 rats in each group.The water environment with small platforms method was used to intervene for 9 days to establish the rat model of insomnia due to corporeal soul restless in the lung.After successful modeling,the Chinese medicine group was given 7.6g·kg-1·d-1 gavage of CDEO,and the Western medicine group was given 0.1 mg·kg-1·d-1 of dexzopiclone tablets;the control group and the model group were gavaged an equal volume of normal saline,for 14 days,and the body weight was measured.The expression levels of Caspase-3,Bcl-2 and Bax in the brain tissues were detected by immunohistochemistry and Western blot.Results:Compared with the control group,the body weight of the model group decreased(P<0.01),the immunohistochemistry results showed that the expression of Bcl-2 in the rat brain tissue significantly decreased(P<0.01),the expression of Caspase-3 and Bax significantly increased(P<0.01),and the ratio of Bcl-2/Bax significantly decreased(P<0.01).Western blot showed that the relative expression of Bcl-2 significantly decreased(P<0.01),and the relative expressions of Bax and Caspase-3 significantly increased(P<0.01).Compared with the model group,the body weight of the Chinese medicine group and the Western medicine group significantly increased(P<0.01),and the results of immunohistochemistry showed that the expression of Bcl-2 in the brain tissue of rats increased(P<0.05),the expression of Caspase-3 and Bax decreased(P<0.05),and the ratio of Bcl-2/Bax significantly increased(P<0.01).Western blot results showed that the relative expression of Bcl-2 increased(P<0.05),while the relative expression of Bax and Caspase-3 significantly decreased(P<0.01).Conclusion:The sleep improvement of cinnamon twig decoction plus eragon bone and oyster shell in the insomnia of rats with insomnia due to corporeal soul restless in the lung may be related with Bcl-2 increase and Caspase-3 and Bax decrease in the brain.