双酶辅助水蒸气蒸馏法提取肉豆蔻精油及其GC-MS成分分析

Extraction of Myristica fragrans Houtt.Essential Oil by Dual Enzyme Assisted Steam Distillation and Its Component Analysis by GC-MS

采用双酶辅助水蒸气蒸馏法提取广西产肉豆蔻的精油,并通过GC-MS对其进行成分分析。正交实验结果表明,最优提取工艺条件为加酶量2%、酶解pH 4.0、酶解温度40℃,在此条件下,肉豆蔻精油的得率为6.50 mL/100 g。利用GC-MS和保留指数法从精油中鉴定出56种化合物,占精油总量的99.71%,主要成分为肉豆蔻醚(16.60%)、甲基丁香酚(14.78%)、桧烯(13.47%)、(+)-柠檬烯(7.29%)、4-萜烯醇(6.75%)、榄香素(6.62%)、γ-松油烯(4.28%)、反式-甲基异丁香酚(3.73%)、β-蒎烯(3.59%)和黄樟素(3.22%)。

Dual enzyme assisted steam distillation is used to extract essential oil from Myristica fragrans Houtt.in Guangxi,and its components are analyzed by GC-MS.The orthogonal experimental results indicate that the optimal extraction process conditions are enzyme addition amount of 2%,enzymatic hydrolysis pH of 4.0 and enzymatic hydrolysis temperature of 40℃.Under these conditions,the yield of essential oil from Myristica fragrans Houtt.is 6.50 mL/100 g.Fifty-six compounds are identified in essential oil by GC-MS and retention index method,accounting for 99.71%of the total essential oil.The main components are myristicin(16.60%),methyleugenol(14.78%),sabinene(13.47%),(+)-limonene(7.29%),terpinen-4-ol(6.75%),elemicin(6.62%),γ-terpinene(4.28%),trans-methyl eugenol(3.73%),β-pinene(3.59%)and safrole(3.22%).