珍稀树种秃叶红豆外植体启动培养的影响因素

Study on Factors Influencing Initial Culture of Explants of Precious Ormosia nuda

本研究以四年生扦插苗为材料,探索不同消毒方式、外植体、培养基对秃叶红豆丛生芽启动培养的影响。结果表明,以当年生带腋芽半木质化嫩枝为最佳外植体;用50 mg/L利福平与50 mg/L氯霉素混合液浸泡30 min,然后用75%乙醇浸泡60 s,最后用0.1%氯化汞二段式浸泡各5 min,其污染率为45.00%,灭菌有效值为22.92%;启动培养的最佳培养基为B5+2.0 mg/L 6-BA+0.1 mg/L NAA。研究表明,外植体、消毒方式、培养基是秃叶红豆丛生芽启动培养的重要影响因素,通过筛选丛生芽启动培养的适宜条件可为建立高效离体再生体系提供理论依据和技术指导。

Effects of different sterilization methods,explants and culture media on the initial culture of clustered buds with 4-year-old cutting seedlings were explored.The results showed that semi-lignified twig with axillary buds was the best explant.The pollution rate was 45.00%,and the effective sterilization value was 22.92%by soaking with 50 mg/L rifampicin and 50 mg/L chloramphenicol mixture for 30 min,then soaking with 75%ethanol for 60 s,and then soaking with 0.1%mercury chloride in two stages for 5 min,respectively.The optimal medium for initial culture was B 5+6-BA 2.0 mg/L+NAA 0.1 mg/L.Study showed that sterilization method,explant,and culture media were the important factors to influence the initial culture of clustered buds of Ormosia nuda.The optimal propagation conditions for the initial culture was screened to provide theoretical basis and technical guidance for the establishment of efficient in vitro regeneration system.