摘要
报道了一种新型的甲胎蛋白检测用无酶型电化学免疫传感器。这种免疫传感器利用金@铂纳米粒子对过氧化氢的高催化性能和石墨烯的高导电性能,增加了电子转移能力和信号放大能力,摆脱了免疫检测中对酶的需要,从而使无酶检测成为可能。这种新型免疫传感器对甲胎蛋白的检测限为0.00003 pg/mL,线性范围为在0.0001~10 pg/mL。
A novel electrochemical immunosensor capable of enzyme-free detection of alpha fetoprotein(AFP)is reported.This immunosensor uses the high catalytic properties of catalyzed gold-platinum nanoparticles against hydrogen peroxide and the high conductive performance of graphene to increase electron transfer capacity and signal amplification,escaping the need for enzymes in immunodetection,thus enabling enzyme-free detection.With a low detection limit at 0.00003 pg/mL,this novel immunosensor worked well over the broad linear range of 0.0001~10 pg/mL.
作者
赵丽芳
于晓菲
滕龙
齐云
刘鹏飞
侯斌
张娟
Zhao Lifang;Yu Xiaofei;Teng Long;Qi Yun;Liu Pengfei;Hou Bin;Zhang Juan(Shandong Institute for Product Quality Inspection,Jinan 250100,China;Shandong Huankeyuan Environmental Engineering Co.,Ltd.,Jinan 250109,China;Binzhou Inspection and Testing Center,Binzhou 256600,China)
出处
《山东化工》
CAS
2024年第1期20-22,29,共4页
Shandong Chemical Industry
基金
食品用洗涤剂中防腐剂的检测技术体系构建(项目编号:2021ZJKY023)。
关键词
甲胎蛋白
无酶型
电化学免疫传感器
alpha fetoprotein
enzyme-free
electrochemical immunosensor