基于氨基化石墨烯-金@铂纳米粒子复合材料的无酶型电化学免疫传感器的构建及应用

Construction and Application of Enzyme-Free Electrochemical Immunosensor Based on Amino-Fossil Graphene-Au@Pt Nanoparticle Composites

报道了一种新型的甲胎蛋白检测用无酶型电化学免疫传感器。这种免疫传感器利用金@铂纳米粒子对过氧化氢的高催化性能和石墨烯的高导电性能,增加了电子转移能力和信号放大能力,摆脱了免疫检测中对酶的需要,从而使无酶检测成为可能。这种新型免疫传感器对甲胎蛋白的检测限为0.00003 pg/mL,线性范围为在0.0001~10 pg/mL。

A novel electrochemical immunosensor capable of enzyme-free detection of alpha fetoprotein(AFP)is reported.This immunosensor uses the high catalytic properties of catalyzed gold-platinum nanoparticles against hydrogen peroxide and the high conductive performance of graphene to increase electron transfer capacity and signal amplification,escaping the need for enzymes in immunodetection,thus enabling enzyme-free detection.With a low detection limit at 0.00003 pg/mL,this novel immunosensor worked well over the broad linear range of 0.0001~10 pg/mL.