儿童支原体肺炎患者血清CCL-2、IL-6、TGF-β_(1)、SP-A变化及与疾病严重程度的关系

Changes of Serum CCL-2,IL-6,TGF-β_(1) and SP-A in Children with Mycoplasma Pneumoniae Pneumonia and Their Relationship with Disease Severity

目的探讨儿童支原体肺炎(MPP)患者血清趋化因子配体2(CCL-2)、白介素-6(IL-6)、转化生长因子-β_(1)(TGF-β_(1))、肺表面活性物质相关蛋白A(SP-A)水平变化及与疾病严重程度的关系。方法纳入100例2019年6月至2022年6月入院且经检测诊断为MPP儿童患者为研究对象(MMP组),根据临床表现及影像学表现分为轻症组(62例)、重症组(38例)。选择同期来院健康体检的儿童患者为参照对象(健康组,100例)。采集两组儿童静脉血样,检测并比较MPP组与健康组儿童血清CCL-2、IL-6、TGF-β_(1)、SP-A水平,并分析其与MPP严重程度的关系,绘制ROC曲线分析单独检测和四者联合检测对MMP严重程度诊断的效能。结果MPP组患儿血清中CCL-2、IL-6、TGF-β_(1)、SP-A水平较健康组升高,且MPP患儿重症组中的水平较轻症组更高,差异有统计学意义(P<0.05);Pearson相关分析显示,CCL-2、IL-6、TGF-β_(1)、SP-A与儿童MPP严重程度呈正相关(P<0.05);ROC曲线结果显示,四者联合检测诊断儿童MPP的AUC为0.940,高于单独检测(P<0.05)。结论CCL-2、IL-6、TGF-β_(1)、SP-A水平在MPP患儿血清中升高,并与MPP严重程度相关,四者联合检测更有利于对MPP严重程度进行临床诊断。

Objective To explore the changes of serum chemokine ligand-2(CCL-2),interleukin-6(IL-6),transforming growth factor-β_(1)(TGF-β_(1))and pulmonary surfactant-associated protein A(SP-A)in children with mycoplasma pneumoniae pneumonia(MPP)and their relationship with disease severity.Methods A total of 100 children with MPP confirmed in the Sanmenxia Hospital of Yellow River were enrolled as MMP group between June 2019 and June 2022.According to clinical manifestations and imaging findings,they were divided into mild group(62 cases)and severe group(38 cases).A total of 100 healthy children during the same period were enrolled as healthy group.The samples of venous blood were collected to detect and compare levels of serum CCL-2,IL-6,TGF-β_(1) and SP-A in MPP group and healthy group,and the relationship between the above indexes and MPP severity was analyzed.The diagnostic efficiency of single indexes and combined detection for MMP severity was analyzed by ROC curves.Results The levels of serum CCL-2,IL-6,TGF-β_(1) and SP-A in MPP group were significantly higher than those in healthy group,and which were higher in severe group than those mild group(P<0.05).Pearson correlation analysis showed that levels of serum CCL-2,IL-6,TGF-β_(1) and SP-A were positively correlated with the severity of MPP(P<0.05).The results of ROC curves analysis showed that area under the curve(AUC)of combined detection in the diagnosis of MPP severity was 0.940,greater than that of single index(P<0.05).Conclusion The levels of serum CCL-2,IL-6,TGF-β_(1) and SP-A are increased in MPP children,and which are correlated with the severity of MPP.The combined detection of the four indexes is more conducive to the clinical diagnosis of MPP severity.