慢性HBV携带者共病抑郁症患者血清miRNA-22的表达

Expression of serum miRNA-22 in patients with comorbid depression and chronic HBV carriage

目的检测慢性乙肝携带者共病抑郁症患者、抑郁症患者、乙型肝炎携带者和健康对照者血清中miRNA-22(miR22)的表达情况。方法收集4组(共病组、抑郁组、乙肝携带组、健康组)共120份人类血清标本。采用荧光定量PCR(探针法)检测人类血清中miR22-3p和miR22-5p的表达。收集基本的社会人口临床特征等资料,并完善汉密尔顿抑郁量表评定。采用Kruskal-Wallis检验、Pearson法和Spearman法进行相关性分析,Bonferroni法对P值进行校正,构建单因素线性回归模型。结果本研究共有120例受试者参与,共病组miR22-5p和miR22-3p的基因表达水平显著高于抑郁组、乙肝携带组与健康组3组(P<0.01)。结论miR22可能通过调控神经递质和靶基因等多个信号通路影响神经元的功能,在慢性HBV携带者共病抑郁症的发病机制中发挥重要作用,miR22的表达水平可以作为乙肝病毒感染相关抑郁的治疗预测指标,提高对乙型肝炎病毒感染和抑郁症患者的早期诊治及防范。

Objective To detect the expression of serum miRNA-22(miR22)in hepatitis B carriers with comorbid depression,depression patients,hepatitis B carriers,and healthy controls.Methods A total of 120 human serum samples were collected from 4groups(comorbid group,depression group,hepatitis B carrier group,and healthy control group).The expression of miR22-3p and miR22-5p in human serum was detected using fluorescence quantitative PCR(probe method).Basic demographic and clinical characteristics were collected,and the Hamilton Depression Scale was used for assessment.Kruskal-Wallis test,Pearson′s method,and Spearman′s method were used for correlation analysis.Bonferroni correction was applied to adjust the P-values,and a univariate linear regression model was constructed.Results A total of 120 participants were included in this study.The gene expression levels of miR22-5p and miR22-3p in the comorbid group were significantly higher than those in the depression group,hepatitis B carrier group,and healthy control group(P<0.01).Conclusion miR22 may play an important role in the pathogenesis of comorbid depression in chronic HBV carriers by regulating neurotransmitters and target genes through multiple signaling pathways.The expression levels of miR22 can serve as predictive indicators for the treatment of depression associated with hepatitis B virus infection,and can improve early diagnosis,treatment,and prevention of hepatitis B virus infection and depression.