快速石蜡切片技术应用于病理检验中的临床价值分析

Analysis of the clinical value of applying rapid paraffin section technique in pathological examination

目的探讨于病理检验中应用快速石蜡切片技术的价值。方法60例行手术治疗且实施病理检验的患者,按照数字随机法分成对照组及观察组,每组30例。对照组样本进行常规处理,观察组样本进行快速石蜡切片技术处理。比较两组患者的切片制作时间,切片质量,诊断准确率,切片制作指标(第一道浸蜡时间、第二道浸蜡时间、透明脱水时间、固定时间),满意度。结果(1)与对照组比较,观察组切片制作时间显著较短,其中对照组切片制作时间为(128.66±23.15)min,观察组切片制作时间为(42.59±4.30)min,具备统计学差异(t=20.021,P=0.000<0.05)。(2)观察组高质量切片率96.67%显著高于对照组的66.67%,低质量切片率3.33%和废片率0显著低于对照组的20.00%、13.33%,具备统计学差异(P<0.05)。(3)与对照组比较,观察组诊断准确率显著较高,其中对照组诊断准确率为80.00%(24/30),观察组诊断准确率为96.67%(29/30),具备统计学差异(χ^(2)=4.043,P=0.044<0.05)。(4)观察组第一道浸蜡时间、第二道浸蜡时间、透明脱水时间、固定时间分别为(60.96±4.24)、(90.82±6.29)、(30.90±1.22)、(60.29±2.38)min,短于对照组的(89.83±5.36)、(154.33±8.38)、(110.83±3.22)、(119.83±2.52)min,具备统计学差异(P<0.05)。(5)对照组患者满意度为73.33%,观察组患者满意度为93.33%,观察组高于对照组,具备统计学差异(P<0.05)。结论在病理检验中应用快速石蜡切片技术,其临床价值显著突出,与常规的样本切片技术比较,具有缩短时间,切片质量高等优势,利于临床对于病理的诊断,为患者的检查争取宝贵时间。

Objective To explore the value of applying rapid paraffin section technique in pathological examination.Methods 60 patients who underwent surgical treatment and underwent pathological examination were divided into an observation group and a control group using a random number table method,with 30 cases in each group.Samples in the control group were treated with routine section technique,while samples in the observation group were treated with rapid paraffin section technique.The section preparation time,section quality,diagnostic accuracy,section preparation indicators(the first and second time of paraffin immersion,transparent dehydration time,and fixed time)were compared between the two groups of patients.Results(i)Compared with the control group,the section preparation time of the observation group was significantly shorter.The section preparation time was(128.66±23.15)min in the control group and(42.59±4.30)min in the observation group,and there was statistical difference(t=20.021,P=0.000<0.05).(ii)The high-quality section rate of 96.67%in the observation group was higher than 66.67%in the control group,while the low-quality section rate of 3.33%and scrap rate of 0 in the observation group were lower than 20.00%and 13.33%in the control group,and there was statistical difference(P<0.05).(iii)Compared with the control group,the diagnostic accuracy of the observation group was significantly higher,with an accuracy of 80.00%(24/30)in the control group and 96.67%(29/30)in the observation group,and there was statistical difference(χ^(2)=4.043,P=0.044<0.05).(iv)The observation group had the first time of paraffin immersion of(60.96±4.24)min,second time of paraffin immersion of(90.82±6.29)min,transparent dehydration time of(30.90±1.22)min,and fixed time of(60.29±2.38)min,which were significantly shorter than(89.83±5.36),(154.33±8.38),(110.83±3.22),and(119.83±2.52)min in the control group,and there was statistical difference(P<0.05).(v)The satisfaction rate of patients in the control group was 73.33%,while the satisfaction rate of patients in the observation group was 93.33%.The observation group was higher than the control group,and there was statistical difference(P<0.05).Conclusion The clinical value of applying rapid paraffin section technique in pathological examination is significantly outstanding.Compared with routine sectioning technique,it has the advantages of shorter section production time and higher section quality,which is conducive to the clinical diagnosis of pathology and gaining valuable time for the examination of patients.