参附黄配方对脓毒症小鼠不同脑区IL-33/ST2轴的影响

Effects of Shenfuhuang Decoction on IL-33/ST2 Axis in Different Brain Regions of Septic Mice

目的 观察参附黄配方对脓毒症小鼠不同脑区白细胞介素-33(IL-33)/ST2轴的影响。方法 C57BL/6小鼠随机分为对照组、脓毒症组和参附黄组。脓毒症组和参附黄组采取盲肠结扎穿孔术(CLP)复制脓毒症模型。参附黄组术后给予每日2次的2.5 g/kg参附黄配方0.5 m L灌胃;脓毒症组和对照组给予每日2次的纯净水0.5 mL灌胃。采用临床分级评价小鼠临床指数,采用神经功能分级评价神经功能指数,采用酶联免疫标记法评价各组小鼠前额叶皮层和海马区IL-33和ST2的含量。结果 与对照组比较,脓毒症组小鼠临床指数显著增高,神经功能指数显著降低(P<0.05);前额叶皮层和海马区IL-33和ST2蛋白表达显著增多(P<0.05)。与脓毒症组相比较,参附黄组临床指数显著降低(P<0.05),神经功能指数显著增高(P<0.05);前额叶皮层和海马区IL-33蛋白表达显著增高(P<0.05),ST2蛋白表达显著减少(P<0.05)。结论 参附黄配方对CLP小鼠临床症状和神经功能具有一定保护作用,其机制可能与调节IL-33/ST2轴有关。

Objective:To investigate the effects of Shenfuhuang Decoction(SFHD)on interleukin-33(IL-33)/ST2 axis in different brain regions of septic mice.Methods:C57BL/6 mice were randomly divided into control group,sepsis group and SFHD group.The sepsis model was established by cecal ligation and puncture(CLP)in the sepsis group and the SFHD group.The SFHD group was given 2.5 g/kg SFHD twice a day by intragastric administration,with an intragastric volume of 0.5 mL.The sepsis group and the control group were given 0.5 mL pure water by gavage twice a day.Clinical grading was used to evaluate the clinical index of mice,neurological function grading was used to evaluate the neurological function index,and enzyme-linked immunosorbent assay was used to evaluate the content of IL-33 and ST2 in the prefrontal cortex and hippocampus of mice in each group.Results:Compared with the control group,the clinical index was increased and neurological function index of the mice was decreased in the sepsis group(P<0.05),and the expressions of IL-33 and ST2 proteins in the prefrontal cortex and hippocampus were significantly increased(P<0.05).Compared with the sepsis group,the clinical index was decreased and neurological function index was increased of the SFHD group(P<0.05),the expressions of IL-33 was significantly increased(P<0.05),and the expression of ST2 protein was significantly decreased in the preforntal and hippocampus(P<0.05).Conclusion:SFHD has a certain protective effects on the clinical symptoms and neurological function of CLP mice,and its mechanism may be related to the regulation of IL-33/ST2 axis.