黄芪建中汤对脾胃虚寒证胃溃疡大鼠炎症因子及HGF/c-Met信号通路的影响

Effects of Huangqi Jianzhong Decoction on Inflammatory Factors and HGF/c-Met Signaling Pathway in Rats with Gastric Ulcer of Spleen-stomach Deficiency and Cold Syndrome

目的观察黄芪建中汤对脾胃虚寒证胃溃疡大鼠炎症因子及HGF/c-Met信号通路的影响。方法将60只大鼠随机分为4组,即正常组、模型组、黄芪建中汤组[黄芪建中汤6.8 g/(kg·d)]、奥美拉唑组[奥美拉唑肠溶胶囊4.2 mg/(kg·d)],每组15只大鼠。正常组除外,其他组采用耗气破气法结合饥饱失常法进行大鼠脾胃虚寒证模型造模,再采用冰醋酸法建立大鼠胃溃疡模型。比较各组大鼠体质量、溃疡指数;HE染色观察大鼠胃组织病理学变化;采用酶联免疫吸附法检测血清中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)含量;免疫组化法检测胃组织中肝细胞生长因子(HGF)、肝细胞生长因子受体(c-Met)表达;荧光定量PCR检测胃组织HGF、c-Met m RNA水平。结果与正常组相比,模型组大鼠体质量降低,溃疡指数升高,IL-6、IL-1β、TNF-α含量升高,HGF升高,HGF、c-Met m RNA升高(P<0.05);与模型组相比,黄芪建中汤组和奥美拉唑组大鼠体质量升高,溃疡指数降低,IL-6、IL-1β、TNF-α降低,HGF升高,HGF、c-Met mRNA升高(P<0.05)。结论黄芪建中汤可以通过抑制炎症因子、调节HGF/c-Met通路的表达促进胃黏膜修复。

Objective:To observe the effects of Huangqi Jianzhong Decoction on inflammatory factors and HGF/c-Met signaling pathway in rats with gastric ulcer of spleen-stomach deficiency and cold syndrome.Methods:60 rats were randomly divided into 4 groups:normal group,model group,Huangqi Jianzhong Decoction group[Huangqi Jianzhong Decoction 6.8 g/(kg·d)]and omeprazole group[omeprazole enteric-coated capsules 4.2 mg/(kg·d)],with 15 rats in each group.Except for the normal group,the other groups were established with the spleen-stomach deficiency cold syndrome model by the method of qi depletion and qi destruction combined with the method of hunger and satiety disorder,and the rat gastric ulcer model was established with glacial acetic acid method.The body weight and ulcer index of rats in each group were compared.HE staining was used to observe the pathological changes of gastric tissue.The levels of IL-6,IL-1βand TNF-αin serum were detected by enzyme-linked immunosorbent assay.The expression of hepatocyte growth factor(HGF)and hepatocyte growth factor receptor(c-Met)in gastric tissue was detected by immunohistochemistry.The mRNA levels of HGF and c-Met in gastric tissue were detected by real-time PCR.Results:Compared with the normal group,the body weight of the model group was decreased,the ulcer index was increased,the contents of IL-6,IL-1β,TNF-αwere increased,HGF was increased,HGF and c-Met mRNA were increased(P<0.05);compared with the model group,the body weight of the rats in Huangqi Jianzhong Decoction group and omeprazole group was increased,the ulcer index was decreased,IL-6,IL-1βand TNF-αwere decreased,HGF,HGF and c-Met mRNA were increased(P<0.05).Conclusion:Huangqi Jianzhong Decoction can promote the repair of gastric mucosa by inhibiting inflammatory factors and regulating the expression of HGF/c-Met pathway.