他扎罗汀对角质形成细胞体外增殖及分泌转化生长因子β1的影响

Tazarotene inhibits proliferation of keratinocyte in vitro and enhances secretion of transforming growth factor-β1

对正常人表皮角质形成细胞(KC)进行原代培养,通过细胞计数、细胞增殖试验(亚甲蓝染色)及ELISA方法检测转化生长因子β1(TGF-β1)的含量等体外观察他扎罗汀对KC的药理作用及其对KC分泌TGF-β1含量的影响。结果新一代受体选择的维A酸药物他扎罗汀可明显抑制KC的生长,具有时间和浓度依赖性特点;且可提升KC分泌TGF-β1的含量。由此可见,他扎罗汀可通过提升KC分泌TGF-β1的含量来进一步抑制KC的增殖。

With the use of primary cultured of keratinocyte from normal person epidermis,to investigate the pharmacological ef-fect of tazarotene for keratinocyte through enumerating the keratinocyte,the test of cellular proliferation and to examine TGF-β1protein content by ELISA;to examine the pharmacological effect of tazarotene for keratinocyte and effect of the secre-tion content of TGF-β1.The results revealed that a new generation of receptor-selective retinoids,tazarotene,obviously inhibited proliferation of keratinocyte in vitro and enhance secretion of transforming growth factor-β1.The studies demonstrated that tazarotene through enhancing secretion of transforming growth factor-β1to suppress keratinocyte proliferation.