双歧杆菌基于菊粉促进普拉梭菌增殖的探究

Bifidobacterium promotes the growth of Faecalibacterium prausnitzii in inulin

普拉梭菌(Faecalibacterium prausnitzii)是人体肠道中常见的微生物,约占整个肠道菌群含量的5%,是肠道中最主要的产丁酸盐菌,对维护肠道健康具有重要作用。它的丰度在患有炎症性肠病和代谢性疾病患者的肠道中显著降低。然而,由于普拉梭菌对氧气极其敏感等特点而限制它的应用发展,寻找一种可替代的手段来增殖普拉梭菌具有重要意义。该文通过在体外建立双歧杆菌和普拉梭菌共培养体系发现双歧杆菌可以显著增加普拉梭菌在菊粉中的生长量及产丁酸盐能力,同时通过16S rRNA测序小鼠粪便菌群也发现灌胃双歧杆菌和菊粉可以在一定程度上增加小鼠肠道中普拉梭菌的丰度,为普拉梭菌在人体肠道中增殖提供理论依据。

Faecalibacterium prausnitzii is a commensal gut bacterium that accounts for about 5%of total bacteria.It is the main butyrate-producing bacterium and plays a vital role in maintaining human health.It has decreased greatly in patients with intestinal disorders and metabolic diseases.However,this species is extremely sensitive to oxygen,which limits the application as a probiotic.Thus,it is pressing to find an alternative strategy to improve its growth.It has established a co-culture system between F.prausnitzii and Bifidobacterium,the results demonstrated that Bifidobacterium could greatly improve the growth of F.prausnitzii and increase the content of butyrate.The 16S rRNA sequencing discovered that Bifidobacterium and inulin can increased the abundance of F.prausnitzii in fecal flora.It has provided a theoretical basis for the proliferation of F.prausnitzii in human intestines.