红枣环磷腺苷的提取工艺优化及应用研究

Study on extraction process optimization and application of cyclic adenosine monophosphate from jujube

该实验对红枣中环磷腺苷的提取工艺和应用进行了研究,通过单因素试验分别考查了料液比、提取时间、乙醇体积分数3个单因素对于环磷腺苷得率的影响,确定了在环磷腺苷得率最高的情况下的各因素的最适条件,并利用得到的提取物制备了乳霜。利用Design-Expert V8.0.6软件进行中心组合设计法设计响应面的实验组,通过实验得到的数据建立模型,得到环磷腺苷的最优提取工艺为:料液比1∶25.42(g∶mL)、提取时间63.51 min、乙醇体积分数61.63%,并对乳霜稳定性进行检测。在最佳提取工艺下,环磷腺苷得率为189.07μg/g,并利用提取物制备得到一款具有良好稳定性的乳霜。

This paper aimed to improve the utilization value of jujube and develop products for deep processing.This research examined the extraction method and application of cyclic adenosine monophosphate(cAMP)from jujube.Using a single-factor experiment,the effects of three parameters on the yield of cAMP were investigated,including solid-liquid ratio,extraction time,and ethanol concentration.The optimal conditions for each ingredient for the highest yield of cAMP were found,and the resulting extract was used to formulate a cream.Using the software Design-Expert V8.0.6 and a central combination design,the experimental group of response surfaces was created.The optimal extraction method for cAMP was determined by establishing a model based on the experimental data,which was 1∶25.42(g∶mL)of the ratio of solids-liquids,63.51 min of extraction time,and 61.63%of the concentration of ethanol,and the cream stability was evaluated.Under the optimal extraction conditions,the yield of cAMP was 189.07μg/g,and a stable cream was formulated with the extract.