应用STR荧光标记分析烟台地区草莓种质资源遗传多样性

Genetic Diversity Analysis of Strawberry Germplasm Resources in Yantai Region Using STR Fluorescent Markers

本研究以烟台地区1份野生草莓种质和6份常规栽培品种为试材,选取已发表具有扩增多态性的11对STR荧光标记引物进行扩增,采用多重荧光毛细管电泳检测,分析了该地区草莓种质的遗传多样性,并构建了其分子身份证.结果表明,用这11对引物从7份草莓种质中共检测到60个STR等位基因位点,平均每对引物5.45个,扩增片段长度在90~270 bp之间,多态信息含量平均为0.676,可用于草莓种质的多态性检测.聚类分析显示7份草莓种质间的遗传相似系数为0.077~0.842,在遗传相似系数为0.257时,7份种质被分为3个类群,W1(野生草莓)与S2(丰香种质)同源性较高.对7份草莓种质进行不同等位基因编码,构建了分子身份证.本研究结果可为烟台地区草莓种质资源鉴定、保护和开发利用提供重要依据.

One wild strawberry germplasm and six conventional cultivars in Yantai region were used as test materials.Their genetic diversity were analyzed through amplifying with 11 pairs of reported STR fluores-cent marker primers which had been verified to have amplification polymorphism and detecting with the multi-ple fluorescence capillary electrophoresis.Their molecular identity cards were also constructed.The results showed that a total of 60 STR allele loci were detected from the 7 strawberry germplasms using the 11 pairs of primers,with the mean of 5.45 loci per pair of primers;the length of the amplification fragments was between 90 and 270 bp,and the average polymorphism information content(PIC)of the primers was 0.676,indicating that these primers could be used for polymorphism detection of strawberry germplasms.Cluster analysis results showed that the genetic similarity coefficient of the 7 strawberry germplasms was 0.077~0.842.When the ge-netic similarity coefficient was 0.257,the 7 germplasms were divided into 3 groups,and the wild germplasm and S2(Fengxiang)had high homology.The molecular identity cards of the seven strawberry germplasm re-sources were constructed by encoding different alleles.These results could provide important references for the identification,protection and development of strawberry germplasm resources in Yantai region.