Hsa_circ_0001459在EB病毒感染者外周血单个核细胞中的表达水平及其临床意义

Expression of hsa_circ_0001459 in peripheral blood mononuclear cells of patients with Epstein-Barr virus infection and its clinical significance

目的检测hsa_circ_0001459在EB病毒(Epstein-Barr virus,EBV)感染者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达水平,判断其对EBV感染者的诊断价值。方法全转录组测序获得EBV感染者PBMC中差异表达circRNAs表达谱,根据∣log2差异倍数∣≥1且P<0.05标准筛选出显著差异的circRNAs,再结合circRNAs组成、长度和引物特异性挑选出高表达的hsa_circ_0001459作为研究对象。采用实时荧光定量PCR方法检测60例EBV感染者和45例健康对照者PBMC中hsa_circ_0001459的相对表达量;受试者工作特征(ROC)曲线和Kappa分析其对EBV感染者的诊断价值。结果与健康对照组相比,hsa_circ_0001459在EBV感染者中表达上调趋势与测序结果一致;其曲线下面积为0.83(95%CI为0.75~0.91),敏感度为0.80(95%CI为0.66~0.89),特异度为0.77(95%CI为0.65~0.86)。Kappa分析结果表明,hsa_circ_0001459与EBV DNA对判断EBV感染有中度一致性(κ=0.51)。结论Hsa_circ_0001459在EBV感染者PBMC中高表达,可能成为EBV感染者的诊断标志物。

Objective To detect the expression of has_circ_0001459 in peripheral blood mononuclear cells(PBMCs)of Epstein-Barr virus(EBV)-infected individuals and evaluate its diagnostic value for EBV infection.Methods The expression profiles of circRNAs in PBMCs of patients with EBV infection were obtained by whole-transcriptome sequencing.Differentially expressed circRNAs with statistical significance were selected based on the criteria of∣log2Fold Change∣≥1 and P<0.05.Based on the composition,length and primer specificity,hsa_circ_0001459 with high expression was selected for further research.The relative expression of hsa_circ_0001459 in PBMCs of 60 patients with EBV infection and 45 healthy people was detected by real-time fluorescent quantitative PCR.Receiver operating characteristic(ROC)curve and Kappa analysis were used to analyze the diagnostic value of hsa_circ_0001459 expression for EBV infection.Results The expression of hsa_circ_0001459 showed an up-regulation trend in patients with EBV infection as compared with that in the healthy people,and the results were consistent with the sequencing results.The area under the ROC curve for screening EBV-infected individuals was 0.83(95%CI:0.75-0.91)with the sensitivity of 0.80(95%CI:0.66-0.89)and the specificity of 0.77(95%CI:0.65-0.86).Kappa analysis indicated that hsa_circ_0001459 was moderately consistent with EBV DNA in the diagnosis of EBV infection(κ=0.51).Conclusions Hsa_circ_0001459 tended to be highly expressed in PBMCs of EBV-infected individuals,which might be uses as a diagnostic marker for EBV infection.