基于UHPLC-Q-Orbitrap HRMS雀嘴茶醇提液的成分及活性研究

Composition and Activity Analysis of Alcohol Extract of Quezui Tea Based on UHPLC-Q-Orbitrap HRMS

基于超高效液相色谱串联四级杆/静电场轨道阱高分辨质谱快速分析雀嘴茶醇提液的成分,对其体外抗氧化活性和酪氨酸酶抑制活性进行研究。结果表明:从雀嘴茶醇提液中鉴定出1 321个代谢物,其中有1 085个代谢物包括11个超类匹配到人类代谢数据库中,含量较高的活性代谢物,包括新甘草苷、扁蓄苷、醋硝香豆素、槲皮素-3-O-葡萄糖苷桂皮鞣质B1等苯丙素类和芳香聚酮类、咖啡因等有机杂环化合物类、阿糖腺苷等核苷酸及其衍生物以及1-咖啡酰-β-D-葡萄糖、3-O-阿魏酰奎尼酸、绿原酸、2-O-咖啡酰熊果苷等咖啡酰类。雀嘴茶醇提液的DPPH·清除率可达93.56%,ABTS^(+)·清除率可达99.04%,OH·清除率可达94.82%,总还原能力随着醇提液中总多酚浓度的增加而升高且效果极为接近阳性对照抗坏血酸;对酪氨酸酶单酚酶和二酚酶的抑制率分别可达81.50%和58.58%。

To explore its active ingredients,this paper analyzed its alcohol extract based on UHPLC-Q-Orbitrap HRMS,and further studied its antioxidant activity and tyrosinase inhibitiory activity.The results showed that 1 321 metabolites were identified from the alcohol extract,of which 1 085 metabolites,including 11 superclasses were matched to the human metabolic database(HMDB).The high content of the active metabolites such as phenylpropanoids and polyketides include neoliquiritin,quercetin 3-arabinoside,acenocoumarol,quercetin3-O-glucoside,epicatechin-(2β→7,4β→8)-epicatechin-(4β→8)-epicatechin,organoheterocyclic compounds include caffeine,nucleosides,nucleotides,and analogues include vidarabine,as well as caffeyl compounds include quercetin 3-O-glucoside,3-O-feruloylquinic acid,chlorogenic acid,and 2-O-caffeoylarbutin.In addition,the scavenging rates of DPPH·,ABTS^(+)·,and ·OH of the Quezui tea alcohol extract can reach 93.56%,99.04%,and 94.82%,respectively.And the total reduction capacity increased with the increase of the total polyphenols concentration of alcohol extract,and the effect was very close to the positive control ascorbic acid.The inhibition rates of monophenolase and diphenolase were 81.50% and 58.58%,respectively.