内皮损伤后血栓模型制备方法的比较

Comparison of Methods for Generating Thrombotic Models after the Endothelial Injury

目的 内皮损伤在深静脉血栓形成中起着重要的作用,旨在比较多种方法制备兔股静脉内皮损伤后血栓模型的效果,为深入研究内皮损伤及深静脉血栓提供良好的实验基础。方法 45只兔随机分为A、B、C 3组,分别采取单纯夹闭法、联合完全结扎法及联合不全结扎法制备股静脉血栓,每组15只,对比建模后1 d、3 d、7 d血管内超声、实时剪切波表现及局部血管内皮病理改变。结果 血管内径及杨氏模量值于造模7 d后出现明显差异(P <0.05)。组间两两比较:造模7 d C组杨氏模量值均较A组及B组增高(P <0.05)。病理证实,造模第7天C组血管内出现一定程度的纤维蛋白机化血栓。结论 夹闭法联合不全结扎法能够产生较为稳定的内皮损伤和血栓形成,该方法可为深入研究内皮损伤后深静脉血栓提供了有力的实验模型基础。

Objective Endothelial injury plays a crucial role in forming deep vein thrombosis.This study aims to compare the effectiveness of various methods for creating rabbit femoral vein thrombotic models after the endothelial injuryso as to provide a solid experimental foundation for further research on the endothelial injury and deep vein thrombosis.Methods Forty-five rabbits were randomly divided into three groups(A,B,C),with 15 cases in each group and subjected to the endothelial injury using the methods of simple clamping,combined complete ligation,and combined incomplete ligation,respectively.The intravascular ultrasonic manifestations and local endothelial pathological changes were compared at 1,3,and 7 days after modeling.Results Significant differences in vascular diameter and Young’smodulus values were observed after 7 days of modeling(P<0.05).In pairwise comparisons between the groups,the Young’smodulus values in group C were significantly higher than those in groups A and B after 7 days of modeling(P<0.05).Pathological examination confirmed the presence of fibr-inoid thrombus in the blood vessels of group C on the seventh day of modeling.Conclusion Combining simple clam-ping and incomplete ligation can produce a relatively stable endothelial injury and thrombus formation.This method provides a robust experimental model for further investigation into deep vein thrombosis after the endothelial injury.