94份谷子核心种质资源分子身份证构建

Establishment of Molecular Identity in 94 Foxtail Millet Varieties

为科学准确的鉴别谷子种质资源,加强对谷子种质资源管理和新品种保护。筛选出33对SSR标记对94份谷子种质资源进行了分子身份证的构建。从分布在谷子9条染色体上的500多对SSR标记中,筛选出33对多态性标记扩增国内外94份谷子种质资源,检测到203个多态性片段,每对标记的等位基因数为3~10,平均为6.2个。标记位点的多态信息含量(PIC)变幅为0.429~0.864,平均为0.740。品种间特异指数差异较大,为63.336~268.523,平均为189.000。结果表明,根据标记的等位基因数确定10对分子标记b185、b260、b224、b103、b225、CAAS1044、b186、b253、b105、CAAS3008组合,即可将94份核心种质资源完全区分开。

To scientifically and accurately identify foxtail millet germplasm resources,strengthen the management of millet germplasm resources and protect new varieties.33 pairs of SSR markers were selected to establish molecular identity cards for 94 foxtail millet germplasm resources.A total of 500 SSR markers covering 9 linkage groups of foxtail millet chromosomes were screened,of which 33 markers showed polymorphic bands and stable amplification,these markers were used to establish molecular ID of 94 foxtail millet varieties.A total of 203 polymorphic bands were obtained.The number of alleles revealed by each marker was 3-10 with an average of 6.2.The polymorphism information content(PIC)values for the SSR loci ranged from 0.429 to 0.864 with an average of 0.740.Ten markers,including b185,b260,b224,b103,b225,CAAS1044,b186,b253,b105 and CAAS3008,were identified to be efficient in the variety ID construction.The IDs of 94 foxtail millet varieties were established by the 10 pairs of markers,which could clear distinguish all the varieties tested.