基于ISSR及SSR标记的苜蓿遗传多样性分析

Genetic Diversity Analysis of Alfalfa Based on ISSR and SSR Markers

探究苜蓿种质资源亲缘关系,为苜蓿品种鉴定和新品种选育提供理论依据。采用ISSR和SSR分子标记方法对30份苜蓿材料进行遗传多样性分析。12条ISSR标记引物共扩增出125条带,多态性条带117条,多态性条带比率(PPB)为93.01%。有效等位基因数(Ne)、Nei′s基因多样性指数(H)和香农多样性指数(I)均值分别为1.4659,0.2813,0.4314;12条SSR标记引物共扩增出152条带,多态性条带144条,多态性条带比率(PPB)为94.05%。有效等位基因数(Ne)、Nei′s基因多样性指数(H)和香农多样性指数(I)均值分别为1.5427,0.3139,0.4701。2种标记遗传相似系数及聚类分析表明,材料Zxy2010p-7900、Zxy2010p-7740可单独分为一类,与其他材料亲缘关系较远;清水紫花苜蓿、陇东紫花苜蓿、甘农4号杂花苜蓿可分为一类,其品种间遗传相似系数较大,亲缘关系较近。2种标记方法客观真实地检测出供试苜蓿种质的亲缘关系。

To explore the genetic relationship of alfalfa germplasm resources and provide theoretical basis for the identification of alfalfa varieties and the breeding of new varieties.Genetic diversity of 30 alfalfa materials was analyzed by ISSR and SSR markers.A total of 125 bands and 117 polymorphic bands were amplified by 12 ISSR labeled primers.The polymorphic band ratio(PPB)was 93.01%.The average effective allele number(Ne),Nei′s gene diversity index(H)and Shannon diversity index(I)were 1.4659,0.2813 and 0.4314,respectively.A total of 152 bands and 144 polymorphic bands were amplified by 12 SSR markers,and the polymorphism band ratio(PPB)was 94.05%.The average effective allele number(Ne),Nei′s gene diversity index(H)and Shannon diversity index(I)were 1.5427,0.3139 and 0.4701,respectively.The genetic similarity coefficient and cluster analysis of the two markers showed that Zxy2010p-7900 and Zxy2010p-7740 could be divided into a separate category and were far related to other materials.Qingshui alfalfa,Longdong alfalfa and Gannong No.4 mixed alfalfa can be divided into one group,the genetic similarity coefficient between the varieties is large,and the relationship is close.The phylogenetic relationship of the tested alfalfa germplasm was objectively determined by the two labeling methods.