显齿蛇葡萄黄酮提取物促光电损伤修复的研究

The Effect of Flavone Extracts from Ampelopsis Grossedentata on Skin Wound Healing After Laser Treatment

为明确显齿蛇葡萄中双黄酮活性成分(二氢杨梅素和槲皮素提取物)促进光电皮肤损伤修复的效果,以点阵二氧化碳激光分别干预大鼠皮肤、体外培养的人皮肤成纤维细胞及角质形成细胞以建立光电皮肤损伤模型,采用二氢杨梅素和槲皮素提取物干预大鼠皮肤创面或损伤细胞,然后评估大鼠皮肤创面愈合情况和炎症因子水平,成纤维细胞活性氧水平以及角质形成细胞迁移功能。结果表明,二氢杨梅素和槲皮素提取物可以降低激光损伤后皮肤成纤维细胞中活性氧及创面组织中炎症因子白介素-6和肿瘤坏死因子-α水平,促进激光损伤后皮肤角质形成细胞迁移,加速激光损伤后皮肤创面的愈合。研究表明,显齿蛇葡萄中双黄酮活性成分(二氢杨梅素和槲皮素提取物组合)通过抑制点阵二氧化碳激光损伤后皮肤炎症反应,促进皮肤创伤修复。

To investigate the effect of the active ingredients of flavones in Ampelopsis grossedentata(dihydromyricetin and quercetin combined extract)on promoting the wound healing of photoelectric skin injury.Skin of rats and both fibroblasts and keratinocytes from human skin were injured by fractional carbon dioxide laser,respectively.The injured skin of rats and cells were treated by dihydromyricetin and quercetin after the laser injury,and then evaluated for skin wound healing and protein levels(interlukin-6 and tumor necrosis factor-α)of rats,reactive oxygen species fibroblasts,and cell migration of keratinocytes.Flavones in Ampelopsis grossedentata(dihydromyricetin and quercetin combined extract)decreased reactive oxygen species induced by laser injury in fibroblasts and proinflammatory factors(interlukin-6 and tumor necrosis factor-α)in skin,accelerated cell migration of keratinocytes,and promoted wound healing.Dihydromyricetin and quercetin combined extract promotes skin wound healing through inhibiting inflammation in laser-injured skin.