嗜热厌氧菌共培养发酵玉米秸秆产氢的研究

A Study on Hydroger Production from Corn Stran by Co-culture of Thermophilic Anaerobic Bacteria

氢能是对环境无害且可以替代化石燃料的可持续性能源。利用嗜热厌氧菌暗发酵木质纤维素生产氢气是一种极具潜力的生物制氢技术,具有清洁、高效和可再生的优势。构建解糖热解纤维素菌和热解糖厌氧杆菌共培养体系,考察两株菌株接种比例、总接种量和底物浓度对玉米秸秆发酵产氢的影响。实验结果表明,在发酵体系初始pH值7.0,培养温度60℃条件下,当解糖热解纤维素菌和热解糖厌氧杆菌接种比例为3∶2,菌种总接种量为6%,秸秆浓度为15 g·L^(-1)时,体系产氢能力最强。此时,发酵体系产氢量累积达到65.6 mL·g-1-秸秆,氢气含量为46.9%,最大产氢速率为1.47 mL·g^(-1)h^(-1)。

Hydrogen energy is a sustainable energy source that is environmentally friendly and can replace fossil fuels.The use of thermophilic anaerobic bacteria for dark fermentation of lignocellulose to produce hydrogen is a highly promising biological hydrogen production technology,with the advantages of cleanliness,efficiency,and renewability.This study constructed a co-culture system of Caldicellulosiruptor saccharolyticus and Thermoanaerobacterium thermosaccharolyticum,and then investigated effects of inoculation ratio of the two strains,total inoculation amount,and substrate concentration on hydrogen production during corn straw fermentation.The experimental results showed that under the conditions of initial pH 7.0 and cultivation temperature of 60℃,hydrogen production capacity of fermentation system was strongest when inoculation ratio of the two strains was 3∶2,total inoculation amount was 6%,and straw concentration was 15 g·L^(-1).Under this condition,the hydrogen production reached 65.6 mL·g-1-straw,and the hydrogen content was 46.9%,the maximum hydrogen production rate achieved 1.47 mL·g^(-1)h^(-1).