番茄疫霉根腐病菌拮抗细菌HP8-1的鉴定及生物防治潜力

Identification and biocontrol potential of antagonist bacteria HP8-1 against Phytophthora root rot of tomato

【目的】探究HP8-1在番茄上的生物防治潜力,为番茄疫霉根腐病的生物防治提供优良生物防治资源。【方法】通过形态学观察、生理生化特性检测并结合16S rRNA序列分析鉴定HP8-1菌株,探讨其对植物病原菌的抑菌效果和生物学功能及在番茄根际的定殖能力,并通过盆栽试验测定其对番茄疫霉根腐病的防治效果和对番茄生长的影响。【结果】HP8-1为多粘类芽孢杆菌(Paenibacillus polymyxa),对多种植物病原菌具有抑菌效果,具有解磷、分解蛋白、分解纤维素、产嗜铁素和产吲哚乙酸等生物学功能;HP8-1在番茄根系和根际土壤具有稳定的定殖能力,尤其是在接种疫霉菌的条件下其定殖量趋于增加并显著高于未接种疫霉菌的条件;HP8-1处理对番茄疫霉根腐病具有明显的防治效果,防治效果达到57.41%;同时,HP8-1处理显著促进番茄植株生长、提高叶绿素含量和根系活力。【结论】HP8-1菌株稳定定殖在番茄根系和根际土壤,防治番茄疫霉根腐病,促进番茄生长,是对番茄疫霉根腐病具有生物防治潜力的资源。

【Objective】In order to explore biocontrol potential of HP8-1 in tomatoes,providing excellent biocontrol resources for the biological control of Phytophthora root rot of tomato.【Method】The strain HP8-1 was identified through morphological observations,physiological and biochemical characteristics and 16S rRNA sequence analysis and explored its antifungal effect on plant pathogens,biological function and its rhizosphere colonization ability in tomato.The control effect of HP8-1 against Phytophthora root rot of tomato and the influence on tomato growth were measured by pot experiments.【Result】The strain HP8-1 was identified as Paenibacillus polymyxa,which has antifungal activity on a variety of plant pathogens and biological functions with decomposing of phosphorus,protein,cellulose,and producing siderophore and indoleacetic acid.HP8-1 has stable colonization ability in tomato roots and rhizosphere soil,especially under the condition of inoculation with Phytophthoa capsica,the colonization density tended to increase and was significantly higher than that without inoculation.In the pot experiment,HP8-1 performed the successful control effect against Phytophthoa root rot of tomato and the control efficiencies was 57.41%.At the same time,the treatment with HP8-1 significantly promoted the growth of tomato,and increased chlorophyll content and root activity.【Conclusion】The strain HP8-1 can survive stably in tomato root and rhizosphere soil,suppress Phytophthora root rot of tomato and promote tomato growth,which is a biocontrol resource with great potential.