海南红树林底泥海洋放线菌的抗菌及抗群体感应活性筛选

Screening of antimicrobial and anti-quorum sensing activities of marine actinomycetes isolated from mangrove sediment in Hainan

采用滤纸片法以紫色色杆菌、大肠杆菌和金黄色葡萄球菌等为指示菌对84株分离自红树林底泥的海洋放线菌进行抗菌及抗群体感应活性筛选,再对高活性菌株进行活性复筛以及薄层层析化学多样性分析。结果显示,菌株HN2-56具有很强的抗群体感应活性及较弱的抗菌活性,且代谢产物较为丰富。对该菌的代谢产物进行初步分离及GC-MS分析,从其活性组分中鉴定出13个可能的化合物,且多个化合物具有一定的生物活性。活性验证试验发现,化合物4(二乙基甲苯甲酰胺)和12(莪术烯醇)具有抗群体感应活性。经16S rDNA序列分析,结合形态观察和系统发育分析,将菌株HN2-56鉴定为链霉菌(Streptomyces chumphonesis)。该菌株在抗群体感应方面有一定的应用潜力,具有进一步研究的价值。

The antibacterial and anti-quorum sensing activities of 84 actinomycetes isolated from mangrove sediment against the indicator bacteria,Chromobactierium violaceum,Escherichia coli and Staphylococcus aureus,were tested by disc diffusion assay.The highly active strains were selected for secondary activity screen and chemical diversity analysis by thin layer chromatography.The results showed that strain HN2-56 had strong anti-quorum sensing activity and weak antibacterial activity,and its metabolites were abundant.The secondary metabolites of this strain were preliminary isolated and analyzed by GC-MS.13 possible compounds were identified and many of them had certain biological activity.The activity verification results showed that compounds 4(diethyl toluamide)and 12(curcumenol)had anti-quorum sensing activity.Through 16S rDNA sequence analysis,morphology observation and phylogenetic analysis,the strain HN2-56 was identified as Streptomyces chumphonesis.This strain has certain application potential in anti-quorum sensing and has the value of further study.