飞龙掌血茎和根不同炮制品对小鼠的急性毒性试验

Experiment on acute toxicity of different processed products of the stem and root of Toddalia asiatica to mice

旨在了解飞龙掌血[Toddalia asiatica(Linn)Lam.]茎和根不同炮制对小鼠急性毒性作用。130只KM小鼠适应性喂养3 d后,随机分为13组,包括溶媒组和12个药物试验剂量组(1个药材茎生品组和5个茎炮制组,1个根生品组和5个根炮制组,其中5种药材炮制品包括水煮制品、水洗制品、水蒸制品、酒蒸制品和酒炙制品)。溶媒组小鼠按40 mL/kg给予纯净水灌胃,给药3次,间隔4~5 h;茎各炮制品试验组小鼠按40 mL/kg灌胃,给药3次,间隔4~5 h;根各炮制品试验组小鼠按30 mL/kg灌胃,给药2次,间隔4~5 h,记录给药后各组小鼠14 d的主要临床症状、体重变化,并统计死亡率。结果表明,飞龙掌血及各炮制品可对小鼠产生急性毒性作用,临床中毒症状有自主活动减少、安静怠动、俯卧和翻正反射消失。炮制品组与溶媒组比较,在14 d内茎和根各炮制品均可致小鼠体重明显降低,且死亡率显著高于溶媒组(P<0.05或P<0.01);而与生品组比较,茎和根的5种炮制品在14 d内对小鼠体重的影响不显著,可有效降低小鼠的死亡率,其中以水煮法效果最佳。水煮、水洗、水蒸、酒蒸、酒炙炮制方法可以减小飞龙掌血茎和根(生品)对小鼠的毒性作用,但对小鼠体脂影响不显著。

The purpose was to understand the acute toxicity of different processing methods of the stems and roots of Toddalia asiatica on mice.After adaptive feeding for 3 days,130 KM mouses were randomly divided into 13 groups,including the solvent control group and 12 drug-treated groups(including 1 group of raw stem product,5 groups of processed stem products,1 group of raw root product and 5 groups of processed root products.Processed products of stems and roots covered 5 categories:Boiled products,washed prod-ucts,steamed products,wine steamed products and wine roasted products).Mice in the solvent group were given pure water at a dose of 40 mL/kg by gavage for 3 times with an interval of 4~5 h.Mice in the drug-treated group of stem and its processed products were giv-en 40 mL/kg by gavage for 3 times with an interval of 4~5 h.Mice in the drug-treated group of root and its processed products were giv-en by gavage at the rate of 30 mL/kg twice,with an interval of 4~5 h.The main clinical symptoms and weight changes of mice in each group were recorded for 14 days after administration,and the mortality rate was counted.The results showed that Toddalia asiatica and its processed products could have acute toxic effects on mice and the clinical symptoms of toxicity included decreased voluntary activi-ty,inactivity,prone position and disappearance of righting reflex.Compared with the solvent control group,the processed products of stems and roots could significantly reduce the weight of mice within 14 d,and the mortality rate was significantly higher than that of the solvent group(P<0.05 or P<0.01).Compared with the raw product group,the five processed products of stems and roots had no sig-nificant effect on the weight of mice within 14 d,and effectively reduced the mortality of mice,and the best effect of reducing toxicity was the boiling method.Boiling,washing,steaming with water,steaming with wine and roasting with wine reduced the toxic effects of raw stems and roots of Toddalia asiatica on mice,but had no significant effects on body fat of mice.