前列腺癌组织中Wnt5a的表达与血管生成拟态的相关性

Correlation Between Wnt5a Expression and Vasculogenic Mimicry in Prostate Cancer Tissues

目的分析前列腺癌Wnt5a与血管生成拟态(VM)的相关性,并评估其与肿瘤干细胞特性和上皮间质转化的关系。方法免疫组织化学法检测50例前列腺癌和50例前列腺增生组织中Wnt5a的表达及前列腺癌组织中CD133、Vimentin和E-cadherin的表达;CD34/PAS双染检测VM的形成。分析组间Wnt5a的表达差异、Wnt5a和VM的临床意义、Wnt5a与VM的相关性及Wnt5a和VM与CD133、Vimentin、E-cadherin的相关性。结果Wnt5a在前列腺癌组织中的表达明显高于前列腺增生组织(P<0.05),Wnt5a的表达与VM正相关(P<0.05),Wnt5a及VM的表达与CD133及Vimentin的表达正相关(P<0.05)。Wnt5a及VM的表达与前列腺癌Gleason评分、输精管侵犯及淋巴转移均正相关(P<0.05),VM与前列腺癌T分期呈正相关(P<0.05)。结论Wnt5a在前列腺癌高表达,并与VM呈正相关,Wnt5a及VM与肿瘤干细胞特性和上皮向间充质转化的标志蛋白的表达具有相关性。

Objective To investigate the correlation of Wnt5a expression and vasculogenic mimicry(VM)in prostate cancer tissues,and analyze their relationships with cancer stem cells(CSCs)characteristics and epithelial–mesenchymal transition(EMT).Methods Immunohistochemistry was conducted to detect the expression of Wnt5a in 50 prostate cancer tissues and 50 benign prostatic hyperplasia tissues.The expression levels of CD133,vimentin,and E-cadherin were detected in the prostate cancer tissues,and CD34/PAS double staining was used to detect VM structures.We analyzed the difference in Wnt5a level between prostate cancer and benign prostatic hyperplasia tissues,the clinical significance of Wnt5a and VM,the relationship of Wnt5a expression and VM,and the relationships of Wnt5a expression and VM with CD133,Vimentin,E-cadherin.Results The expression of Wnt5a was significantly higher in prostate cancer tissues than in benign prostatic hyperplasia(P<0.05).A positive correlation was observed between Wnt5a expression and VM(P<0.05).The expression levels of Wnt5a and VM were positively correlated with those of CD133 and vimentin(P<0.05).Wnt5a expression and VM were positively correlated with Gleason score,vas deferens invasion and lymphatic metastasis(P<0.05)of prostate cancer,and VM was also positively correlated with T stage of prostate cancer(P<0.05).Conclusion The expression level of Wnt5a in prostate cancer tissues is elevated and positively related with VM formation.Wnt5a expression and VM are correlated with cancer stem cells characteristics and the expression of epithelial–mesenchymal transition marker proteins.