鹰嘴豆种子代谢产物的GC-MS分析

Multivariable comparative analysis of chickpea seed metabolites based on GC-MS

【目的】研究鹰嘴豆品种之间代谢物质的差异,利用GC-MS技术对鹰嘴豆初级代谢产物进行代谢组学分析,为鹰嘴豆产品开发提供理论基础。【方法】利用GC-MS技术及热图聚类,比较分析10个鹰嘴豆品种种子的代谢产物。【结果】检测到146种化合物,主要包括:机酸类、脂肪酸类、氨基酸类、糖类、醇类、酚类以及含氮类七大类化合物。其中8个品种针对每一大类均含有相同的化合物,而D2016-10和YZD80则积累自身特定的化合物,与其他8个品种相同累积的化合物明显偏少。D2016-10和YZD802个品种所积累化合物情况完全不同,存在各自代谢产物的特异性。按化合物的Q值将10个鹰嘴豆品种分为4小类。【结论】10个不同鹰嘴豆品种均含有大量的酸类、糖醇类以及含氮类等几大类化合物,氨基酸类及其衍生物分布相对较少。

【Objective】To explore the differences of metabolites among different chickpea varieties by using GC-MS analysis combined with cluster analysis and to analyze their non targeted metabonomics,so as to provide a theoretical basis for the development of chickpea food,health products and drugs.【Methods】The metabolites of 10 chickpea seeds were analyzed by GC-MS.【Results】146 compounds were detected from the tested materials,mainly including organic acids,fatty acids,amino acids,sugars,alcohols,phenols and nitrogen-containing compounds.After cluster analysis,it was found that the all the eight varieties contained the same distribution of some compounds for each major category of compounds,but D2016-10 and YZD80 accumulated their own specific content of compounds,while the content of the same accumulated compounds of the other eight varieties was significantly less.However,the accumulation of these D2016-10 and YZD80 two varieties was completely different,so they had their own specificity.Divide 10 chickpea varieties into 4 subcategories based on their Q values of compounds.【Conclusion】In general,the 10 different chickpea varieties contain a large number of acids,sugar alcohols and nitrogen-containing compounds,and the distribution of amino acids and their derivatives is relatively small.