FAM83A与β-catenin在宫颈病变组织中的表达差异

Differential expression of FAM83A andβ-catenin in cervical lesions

目的探讨83序列相似成员A(FAM83A)与β-连环蛋白(β-catenin)在宫颈病变组织中的表达差异及潜在临床意义。方法利用UALCAN和GEPIA2.0在线数据库分析FAM83A在正常宫颈和宫颈鳞癌(CSCC)中的表达差异及FAM83A表达与CSCC患者预后的关系,使用LinkedOmics数据库分析FAM83A mRNA共表达基因,利用R语言进行KEGG富集分析。免疫组化方法检测60例正常宫颈组织、80例低级别鳞状上皮内病变(LSIL)、90例高级别鳞状上皮内病变(HSIL)、70例CSCC中FAM83A与β-catenin表达情况。分析FAM83A、β-catenin表达与临床病理特征之间的关系及FAM83A与β-catenin表达的相关性。结果UALCAN数据库分析显示FAM83A在CSCC组织中高表达,GEPIA 2.0数据库分析提示FAM83A高表达者预后不良。LinkedOmics数据库进行KEGG富集分析提示FAM83A的表达与Wnt/β-catenin信号通路的异常激活呈正相关。FAM83A在CSCC中的表达率高于LSIL和正常宫颈组织(P<0.001),而与HSIL相比差异无统计学意义(P=0.401);FAM83A的表达与年龄无相关性(P=0.231),与分化程度(P=0.001)和临床分期(P=0.038)的相关性差异有统计学意义。β-catenin在CSCC中的异常表达率高于LSIL和正常宫颈组织(P<0.001),而与HSIL相比差异无统计学意义(P=0.734);β-catenin的表达与年龄无关(P=0.088),与分化程度(P=0.001)和临床分期(P<0.001)有关,FAM83A与β-catenin表达具有正相关性(P<0.05)。结论FAM83A与β-catenin在HSIL及CSCC组织中均高表达,两者之间表达存在正相关性。FAM83A的高表达与CSCC患者预后存在一定的相关性,可作为判断CSCC预后的潜在标志物。

Objective To investigate the expression difference and potential clinical significance of 83 sequence similar member A(FAM83A)andβ-catenin in cervical lesions.Methods UALCAN and GEPIA2.0 online databases were used to analyze the difference of FAM83A expression in normal cervix and cervical squamous cell carcinoma(CSCC)and the relationship between FAM83A expression and the prognosis of CSCC patients,LinkedOmics database was used to analyze FAM83A mRNA co-expression genes,and R language was used for KEGG enrichment analysis.Immunohistochemistry was used to detect FAM83A andβ-catenin expression in 60 cases of normal cervix,80 cases of low-grade squamous intraepithelial lesion(LSIL),90 cases of high-grade squamous intraepithelial lesion(HSIL)and 70 cases of cervical squamous cell carcinoma(CSCC).The relationship between FAM83A,β-catenin expression and clinicopathological features and the correlation between FAM83A andβ-catenin expression were analyzed.Results UALCAN database analysis showed that FAM83A was highly expressed in CSCC tissues,and GEPIA 2.0 database analysis suggested that those with high FAM83A expression had a poor prognosis.LinkedOmics database performing KEGG enrichment analysis suggested that expression of FAM83A was positively correlated with aberrant activation of Wnt/β-catenin signaling pathway.The expression rate of FAM83A in CSCC was higher than that in LSIL and normal cervical tissues(P<0.001),but there was no significant difference compared with HSIL(P=0.401);the expression of FAM83A was not correlated with age(P=0.231),but was significantly different from the correlation with differentiation(P=0.001)and clinical stage(P=0.038).The abnormal expression rate ofβ-catenin in CSCC was higher than that in LSIL and normal cervical tissues(P<0.001),but there was no significant difference compared with HSIL(P=0.734);the expression ofβ-catenin was not related to age(P=0.088),related to differentiation(P=0.001)and clinical stage(P<0.001),and FAM83A was positively correlated withβ-catenin expression(P<0.05).Conclusion FAM83A andβ-catenin are highly expressed in both HSIL and CSCC tissues,and there is a positive correlation between the expression of FAM83A andβ-catenin.The high expression of FAM83A has some correlation with the prognosis of CSCC patients and can be used as a potential marker to determine the prognosis of CSCC.