m^(6)A“阅读器”YTHDF1在OSCC中的预后价值

Prognostic value of m^(6)A“reader”YTHDF1 in OSCC

目的探讨YTHDF1在口腔鳞状细胞癌(OSCC)中的表达水平与临床病理特征之间的相关性及其潜在的预后价值。方法采用免疫组化(IHC)检测132例OSCC组织及66例癌旁组织中YTHDF1的表达,用免疫印迹法(Western blot)检测OSCC细胞系中YTHDF1蛋白的表达。采用卡方检验分析YTHDF1与临床病理特征的相关性。Kaplan-Meier和Cox因素分析影响患者生存时间因素并绘制YTHDF1基因的生存曲线,评价其潜在的临床意义。结果YTHDF1在OSCC组织中的表达高于癌旁组织(P<0.001),YTHDF1蛋白在OSCC细胞系中的表达较正常口腔上皮角质细胞增高(P<0.001)。YTHDF1的表达与OSCC患者的TNM分期和T分期相关(P<0.05),并且YTHDF1高表达患者较低表达患者生存时间更短(P<0.001)。结论YTHDF1高表达与患者预后不良有关,YTHDF1可能是OSCC治疗的一个潜在靶点。

Objective To investigate the correlation between the expression level of YTHDF1 in oral squamous cell carcinoma(OSCC)and clinicopathologic features and its potential prognostic value.Methods The expression of YTHDF1 in 132 OSCC tissues and 66 paracancerous tissues was detected by immunohistochemistry(IHC),and the expression of YTHDF1 protein in OSCC cell lines was detected by Western blot.The correlation between YTHDF1 and clinicopathological features was analyzed by chi-square test.Kaplan-Meier and Cox factors were used to analyze the factors affecting the survival time of the patients and draw the survival curves of the YTHDF1 gene to evaluate its potential clinical significance.Results The expression of YTHDF1 in OSCC tissues was higher than that in paracancerous tissues(P<0.001),and the expression of YTHDF1 protein increased in OSCC cell lines compared with normal oral epithelial keratinocytes(P<0.001).The expression of YTHDF1 was correlated with the TNM stage and T stage of patients with OSCC(P<0.05),and the patients with high expression of YTHDF1 had a shorter survival time compared with those with low expression(P<0.001).Conclusion High expression of YTHDF1 may be associated with poor patient prognosis and YTHDF1 may be able to serve as a target for OSCC treatment.