黄蜀葵花提取物中总黄酮与总多酚含量测定及体外抑菌与抗氧化活性研究

Determination of Total Flavonoids and Total Polyphenols in Abelmoschi corolla Extracts and Their in vitro Antibacterial and Antioxidant Activities

采用比色法测定了不同产地黄蜀葵花提取物(Abelmoschi corolla extracts,ACE)中的总黄酮和总多酚含量,通过琼脂打孔法和微量稀释法研究了ACE的体外抑菌活性,并通过DPPH法、ABTS法和FRAP法评价了ACE的抗氧化活性。结果表明:不同产地ACE中总黄酮和总多酚含量差别不大,安徽ACE中的总黄酮含量最高,为(4.68±0.37)mg·g^(-1),陕西ACE中的总多酚含量最高,为(1.48±0.11)mg·g^(-1);ACE具有一定的体外抑菌活性,能显著抑制变异链球菌的生长,且呈浓度依赖性,最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为3 mg·mL^(-1)和6 mg·mL^(-1);ACE具有一定的抗氧化活性,能够清除DPPH、ABTS等自由基,且在一定浓度范围内呈量效关系。

In this paper,we determined the contents of total flavonoids and total polyphenols in Abelmoschi corolla extracts(ACE)from different origins by colorimetry.Moreover,we studied antibacterial activity in vitro of ACE by agar punching method and microdilution method.Furthermore,we evaluated the antioxidant activity of ACE by DPPH,ABTS,and FRAP methods.The results show that,there is a negligible difference in the contents of total flavonoids and total polyphenols in ACE from different origins.The content of total flavonoids in ACE from Anhui province is the highest at(4.68±0.37)mg·g^(-1),while the content of total polyphenols in ACE from Shaanxi province is the highest at(1.48±0.11)mg·g^(-1).ACE has certain antibacterial activity in vitro and can significantly inhibit the growth of Streptrococcus mutans in a concentration dependent manner.The minimum inhibitory concentration(MIC)and the minimum bactericidal concentration(MBC)are 3 mg·mL^(-1) and 6 mg·mL^(-1),respectively.ACE has certain antioxidant activity and can scavenge free radicals such as DPPH and ABTS,with a dose-response relationship within a certain concentration range.