链霉菌FJS31-2外分泌物的挖掘及活性分析

Excavation and Activity Analysis of Extracellular Secretion from Streptomyces sp.FJS31-2

通过培养基优化、核糖体工程对链霉菌(Streptomyces sp.)FJS31-2外分泌物产生条件进行了优化,利用高分辨质谱(HRMS)技术对其主要活性产物的成分进行了初步分析,采用磺酰罗丹明B(SRB)比色法测定了外分泌物对肝癌细胞MHCC97H的抑制活性。结果表明,经链霉素多代胁迫结合培养基优化诱导链霉菌FJS31-2产生了分子式为C 28 H 40 O 2的四环萜类化合物,该化合物可抑制肝癌细胞MHCC97H的生长,并呈浓度依赖性。为从链霉菌FJS31-2获取新型抗生素奠定了技术和物质基础。

In this paper,we optimized the generation conditions of extracellular secretion from Streptomyces sp.FJS31-2 through medium optimization and ribosome engineering.Moreover,we preliminarily analyzed the main active products via HRMS technology and measured the inhibition activity of the extracellular secretion against hepatoma cell line MHCC97H via SRB colorimetry.The results show that Streptomyces sp.FJS31-2 is induced by multi-generation stress of Streptomycin combined with medium optimization to produce tetracyclic terpenoids with molecular formula C 28 H 40 O 2,which can inhibit the growth of hepatoma cell line MHCC97H in a concentration dependent manner.This paper lays the technical and material foundation for obtaining new antibiotics from Streptomyces sp.FJS31-2.