miR-15a-5p在乳腺癌耐药与敏感患者中的差异分析及耐药价值预测

Differenceanalysis and prediction of drug resistance value of miR-15a-5p in patients with breast cancer resistant and sensitive groups

目的探讨miR-15a-5p在多西他赛治疗敏感组和耐药组中的表达差异及对临床预后的意义。方法运用TCGA数据库分析miR-15a-5p在恶性肿瘤中的表达情况。收集2019年1月至2020年1月在本院治疗的30例对多西他赛耐药的乳腺癌患者为耐药组,收集同期治疗的30例对药物敏感的乳腺癌患者为敏感组,通过q-PCR荧光定量法检测患者癌组织中miR-15a-5p的表达水平,探讨miR-15a-5p与乳腺癌耐药、分型的关联情况。绘制ROC曲线分析miR-15a-5p对患者预后的预测价值以及miR-15a-5p对乳腺癌患者耐药诊断价值。结果通过泛癌分析发现,miR-15a-5p在乳腺癌等多种癌症中显著高表达。q-PCR结果显示,多西他赛化疗敏感组的miR-15a-5p的表达水平高于耐药组的表达水平(P<0.05)。临床统计分析发现miR-15a-5p与ER(雌激素受体)、PR(孕激素受体)的表达呈负相关,而与人表皮生长因子受体-2的表达无明显关联性。结论miR-15a-5p在多西他赛耐药患者的肿瘤组织中的表达水平明显低于敏感组,且miR-15a-5p在乳腺癌的高表达与乳腺癌的分子分型、预后相关。miR-15a-5p可能参与了乳腺癌的免疫调节最终导致了乳腺癌患者多西他赛耐药。

Objective To investigate the expression difference of miR-15a-5p in docetaxel sensitive group and drug-resistant group and its significance for clinical prognosis.Methods TCGA database was used to analyze the expression of miR-15a-5p in malignant tumors.30 docetaxel-resistant breast cancer patients treated in the hospital from 2019 to 2020 were collected as the drug-resistant group,and 30 drug-sensitive breast cancer patients treated in the same period were collected as sensitive group,and the expression level of miR-15a-5p in cancer tissues of thepatients were detected by q-PCR fluorescence quantification method.To investigate the association between miR-15a-5p and drug resistance and typing of breast cancer.ROC curve was drawn to analyze the predictive value of miR-15a-5p on patient prognosis and the diagnostic value of miR-15a-5p on drug resistance in breast cancer patients.Results Pan-cancer analysis showed that miR-15a-5p was highly expressed in breast cancer and other cancers.q-PCR results showed that the expression level of miR-15a-5p in docetaxel chemotherapy sensitive group was higher than that in drug resistant group(P<0.05).Clinical statistical analysis showed that miR-15a-5p was negatively correlated with the expression of ER(estrogen receptor)and PR(progesterone receptor),but had no significant correlation with the expression of human epidermal growth factor receptor-2.Conclusion The expression level of miR-15a-5p in tumor tissues of docetaxel resistant patients is significantly lower than that of sensitive group,and the high expression of miR-15a-5p in breast cancer is closely related to the molecular typing and prognosis of breast cancer.MiR-15a-5p may be involved in immune regulation of breast cancer and ultimately lead to docetaxel resistance in breast cancer patients.