不同AM真菌处理滇重楼中9种核苷和碱基含量分析

Analysis of the Contents of Nine Nucleosides and Bases in Paris polyphylla var.yunnanensis Treated with Different AM Fungi

目的:研究不同丛枝菌根(AM)真菌对滇重楼产量和品质的影响,为筛选滇重楼的优良AM真菌株提供科学依据。方法:以灭菌土壤为生长基质,于室温盆栽条件下对滇重楼新鲜根茎与28种AM真菌共同培养后采样,选取浸泡于FAA固定液中滇重楼根系30条,采用Philips J M等的方法染色、制片、镜检,根据Trouvelot等的方法统计菌根侵染率,以及用HPLC测定和比较分析各处理组滇重楼根茎中核苷(胞苷、尿苷、鸟苷、胸苷、腺苷、2′-脱氧腺苷)和碱基(尿嘧啶、鸟嘌呤、腺嘌呤)的含量。结果:多数处理组滇重楼AM真菌侵染率均有所提高,其中Ga、Asc和Asp处理组的浸染率最显著(P<0.05);不同AM真菌对滇重楼根茎中核苷和碱基含量的影响有所差异;滇重楼根茎中核苷和碱基的积累可能同时存在协同和竞争2种效应。结论:施加外源AM真菌对滇重楼根内AM真菌的侵染率具有调控(增减)作用,在人工栽培过程中接种Sde、Gd、Sca、Spe、Svi、Pb等6种优势AM真菌,能有效促进滇重楼的品质。

Objective:To provide scientific basis for screening excellent AM fungi strains of Paris polyphylla var.yunnanensis,the effects of different arbuscular mycorrhizal(AM)fungi on the yield and quality of P.polyphylla var.yunnanensis were investigated.Methods:The fresh rhizomes of P.polyphylla var.yunnanensis were cocultured with 28 kinds of AM fungi in pot culture at room temperature with sterilized soil as growth medium.After sampling,30 roots of P.polyphylla var.yunnanensis were soaked in FAA,and the Philips J M method was used for staining,sectioning and microscopic examination,the mycorrhizal infection rate was calculated according to Trouvelot's method.In addition,the contents of nucleosides(cytidine,uridine,guanosine,thymidine,adenosine,2'-deoxyadenosine)and bases(uracil,guanine,adenine)in rhizomes of P.polyphylla var.yunnanensis were determined and compared by HPLC.Results:The infection rates of AM fungi of P.polyphylla var.yunnanensis in most treatment groups were increased,and the infection rates of Ga,Asc and Asp treatment groups were significantly increased.In addition,different AM fungi had different effects on the content of nucleosides and bases in rhizomes;The accumulation of nucleosides and bases in the rhizome of P.polyphylla var.yunnanensis may have both synergistic and competitive effects.Conclusion:Exogenous AM fungi can regulate(increase or decrease)the infection rate of AM fungi in the roots of P.polyphylla var.yunnanensis.Inoculation of Sde、Gd、Sca、Spe、Svi、Pb during artificial cultivation brasilianum,which can effectively promote the quality of P.polyphylla var.yunnanensis.