间作黄芪对当归根际土壤微生物的影响

Effect of Astragalus membranaceus Intercropping on Soil Microorganisms in the Rhizosphere of Angelica sinensis

目的:探讨根际土壤微生物对中药材种植模式的响应方式。方法:以间作黄芪及单作下当归根际土壤为研究对象,基于高通量测序技术,分析土壤微生物在不同季节及种植模式下群落组成及功能的差异。结果:间作下Chao1指数、ACE指数高于单作,变形菌门、放线菌门、酸杆菌门、绿弯菌门和芽单胞菌门为间作下的优势菌门,芽单胞菌门的相对丰度在间作下高于单作。11月间作下γ变形菌纲、7月间作下全噬菌纲的丰度显著高于单作。KEGG功能预测表明,在不同时间及间作下根际土壤细菌与细胞过程、环境信息处理、遗传信息处理、代谢功能下属的23类二级功能基因的相对丰度存在显著差异;参与氮循环的功能基因K00371(narH/narY/nxrB)、(K00374 narI/narV)等OTU数在间作下显著高于单作;不同门、纲在间作下的共生网络比单作下连接紧密且更复杂。结论:当归间作黄芪影响土壤细菌群落组成,增加其丰富度及功能的丰富性,影响土壤氮循环能力,促进土壤微生物间的交互作用。

Objective:In order to explore the response mode of rhizosphere soil microorganisms to the planting mode of Chinese medicinal materials.Methods:The research objects were intercropping Astragalus membranaceus and monocropping Angelica sinensis rhizosphere soil.Based on high-throughput sequencing technology,the differences in community composition and function of soil microorganisms in different seasons and planting patterns were analyzed.Results:The results showed that the Chao1 index and ACE index under intercropping were higher than those of monocropping.Proteobacteria,Acidobacteria,Actinobacteria,Chloroflexi and Gemmatimonadetes were the dominant phylums under intercropping,and the relative abundance of Gemmatimonadetes under intercropping was higher than that under monocropping.The abundance ofγ-Proteobacteria in November and the Homophage in July were significantly higher than that in monocropping.KEGG function prediction showed significant differences in the relative abundance of 23 secondary functional genes under rhizosphere soil bacteria and cell processes,environmental information processing,genetic information processing,and metabolism under different time and intercropping conditions.The OTU numbers of functional genes K00371(narH/narY/nxrB)and(K00374 narI/narV)involved in nitrogen cycling were significantly higher than those in monocropping.Intercropping co-occurrence networks of different phylums and classes are more closely connected and complex than those under monocropping.Conclusion:Angelica sinensis intercropping Astragalus membranaceus affects soil bacterial community composition,increase its richness and functional richness,affects soil nitrogen cycling capacity,and promotes soil microbial interactions.