基于UHPLC-Q-TOF-MS/MS分析猪牙皂中化学成分

Analysis of Chemical Constituents in Gleditsiae Fructus Abnormalis Based on UHPLC-Q-TOF-MS/MS

目的 建立液质联用分析方法全面、快速识别猪牙皂中的化学成分。方法 应用超高液相色谱-四极杆飞行时间串联质谱联用技术(UHPLC-Q-TOF-MS/MS)对猪牙皂提取物进行成分分析;其流动相为0.1%甲酸(A)-乙腈(B),梯度洗脱,流速0.3mL·min^(-1)。在负离子模式下获得精确的分子离子质量及二级碎片信息,结合数据库及对照品进行结构鉴定。结果共筛选出78种化合物(黄酮类化合物32个,酚酸类化合物15个,木脂素类22个,单萜酸类化学成分9个),潜在新化合物14个。结论 本研究全面分析了猪牙皂的化学成分,为该药物药效物质基础和质量标准的研究提供了依据。

OBJECTIVE To Establish a liquid chromatography-mass spectrometry analysis method to comprehensively and quickly identify the chemical components in Gleditsiae Fructus Abnormalis.METHODS The ultra-high liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS) was applied for the composition analysis of Gleditsiae Fructus Abnormalis extract;its mobile phase was 0.1% formic acid(A)-acetonitrile(B) with gradient elution at a flow rate of 0.3 mL·min^(-1).Accurate ion mass and secondary fragmentation information were obtained in negative ion mode,combined with databases and standard reference for structure elucidation.RESULTS A total of 78compounds(32 flavonoids,15 phenolic acids,22 lignans and 9 chemical constituents of monoterpenoid acids) with 14 potential new compounds were screened.CONCLUSION This study comprehensively analyzes the chemical composition of Gleditsiae Fructus Abnormalis,and provides a basis for the investigation of the pharmacodynamic basis and quality standards of this drug.