非小细胞肺癌组织EMSY、PIDD表达与同源重组修复基因的相关性及其临床意义

The correlation and clinical significance of EMSY and PIDD expression with homologous recombination repair genes in non-small cell lung cancer tissue

目的研究非小细胞肺癌(NSCLC)组织中EMSY转录抑制因子(EMSY)、p53诱导的死亡结构域蛋白1(PIDD)表达与同源重组修复基因表达的相关性及临床意义。方法选择2022年1月—2023年4月河北北方学院附属第一医院呼吸与危重症医学科诊治NSCLC患者80例。采用实时荧光定量PCR检测癌组织及癌旁组织中EMSY、PIDD表达与同源重组修复基因人乳腺癌易感基因1(BRCA1)、切除修复交叉互补基因1(ERCC1),核糖核苷酸还原酶亚单位1(RRM1)表达。Pearson相关分析EMSY、PIDD表达与同源重组修复基因表达的相关性;分析EMSY、PIDD表达与NSCLC临床病理相关参数的关系及在NSCLC诊断中的价值。结果NSCLC癌组织中EMSY、PIDD、BRCA1、ERCC1、RRM1 mRNA相对表达量均高于癌旁组织(t/P=30.176/<0.001,27.821/<0.001,25.075/<0.001,16.680/<0.001,25.610/<0.001)。NSCLC癌组织中EMSY、PIDD mRNA与BRCA1、ERCC1、RRM1 mRNA表达均呈正相关(r/P=0.654/<0.001,0.712/<0.001,0.584/<0.001;0.724/<0.001,0.661/<0.001,0.563/<0.001)。低分化程度、有淋巴结转移及TNM分期Ⅲ期NSCLC癌组织EMSY、PIDD mRNA表达分别显著高于高中分化程度、无淋巴结转移及TNM分期Ⅰ~Ⅱ期NSCLC癌组织(t/P=13.693/<0.001,13.380/<0.001,12.197/<0.001;10.289/<0.001,11.130/<0.001,9.405/<0.001)。EMSY、PIDD mRNA及两项联合诊断NSCLC的AUC分别为0.834、0.802、0.906,两项联合诊断的AUC大于单一指标(Z=4.751、5.257,P均<0.001)。结论EMSY、PIDD在NSCLC癌组织中表达升高,与同源重组修复基因表达及不良临床病理特征有关,两者联合有助于NSCLC的诊断。

Objective To study the correlation and clinical significance between the expression of EMSY transcription inhibitor(EMSY),p53 induced death domain protein 1(PIDD),and homologous recombination repair genes in non-small cell lung cancer(NSCILC)tissues.Method Eighty patients with NSCLC were treated by the Respiratory and Critical Care Medicine Department of the First Affiliated Hospital of Hebei North University from January 2022 to April 2023.Real time fluorescent quantitative PCR was used to detect the expression of EMSY,PIDD and homologous recombination repair gene human breast cancer susceptibility gene 1(BRCA1),excision repair cross complementary gene 1(ERCC1),ribonucleotide reductase subunit 1(RRM1)in cancer tissues and adjacent tissues.Pearson correlation analysis of the correlation between EMSY,PIDD expression and homologous recombination repair gene expression;Analyze the relationship between EMSY,PIDD expression and clinical pathological parameters related to NSCLC,and their value in the diagnosis of NSCLC.Results The relative expression levels of EMSY,PIDD,BRCA1,ERCC1,and RRM1 mRNA in NSCLC cancer tissues were higher than those in adjacent tissues(t/P=30.176/<0.001,27.821/<0.001,25.075/<0.001,16.680/<0.001,25.610/<0.001).The expression of EMSY and PIDD mRNA in NSCLC cancer tissue is positively correlated with BRCA1,ERCC1,and RRM1 mRNA(r/P=0.654/<0.001,0.712/<0.001,0.584/<0.001;0.724/<0.001,0.661/<0.001,0.563/<0.001).The expression of EMSY and PIDD mRNA in low differentiation,lymph node metastasis,and TNM stage III NSCLC cancer tissues was higher than that in high differentiation,no lymph node metastasis,and TNM stage I-II NSCLC cancer tissues(t/P=13.693/<0.001,13.380/<0.001,12.197/<0.001;10.289/<0.001,11.130/<0.001,9.405/<0.001).The AUC of EMSY,PIDD mRNA,and two combined diagnoses for NSCLC were 0.834,0.802,and 0.906,respectively.The AUC of the two combined diagnoses was greater than that of a single indicator(Z=4.751,5.257,P<0.001).Conclusion The increased expression of EMSY and PIDD in NSCLC cancer tissue is related to the expression of homologous recombination repair genes and adverse clinical and pathological features.The combination of the two is helpful for the diagnosis of NSCLC.